LABORATORY VB

Paper Code: 
DMBL 502B
Credits: 
02
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course the learner will:

CO 44B: Assess the production of mushrooms

CO 45B: Isolate and identify industrially important microorganisms

CO 46B: Analyze the production of fermented products

CO 47B: Plan and apply the knowledge gained  on Digestion of DNA using restriction enzymes agarose gel electrophoresis

CO 48B: Design and plan experiments for Ligation of DNA fragments and Isolation of plasmid DNA

Hands-on practical, Interactive Lectures,

Discussion,

Demonstrations, Simulations

Class test, Semester end examinations, Hands-on practical assessment

 

 

CONTENTS

  1. Isolation of industrially important microorganisms
    1. amylase producing microorganism
    2. lipase producing microorganism
  2. Isolation of antibiotic producing microorganism from soil
  3. Production of alcohol.
  4. Citric Acid fermentation
  5. Mushroom Production
  6. Digestion of DNA using restriction enzymes and analysis by agarose gel electrophoresis.
  7.  Ligation of DNA fragments
  8.  Isolation of plasmid DNA.

 

 

Academic Year: