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LABORATORY VB
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO48B : Use and relate the Plant tissue culture Laboratory, Operation of Laminar Air Flow Hood and Aseptic manipulation techniques. CO49B : Articulate and corelate the different methods of Callus Initiation, Multiple shoot proliferation, Callus differentiation and organogenesis. CO50B : Analyze and apply the knowledge gained on Digestion of DNA using restriction enzymes agarose gel electrophoresis CO51B : Design and plan experiments for Ligation of DNA fragments, Isolation of plasmid DNA
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Hands-on practical, Interactive Lectures, Discussion, Demonstrations, Simulations |
Class test, Semester end examinations, Hands-on practical assessment |
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1. Introduction to Plant Tissue Culture and lay out of Plant tissue culture Laboratory
2. Operation of Laminar Air Flow Hood.
3. Aseptic manipulation techniques
4. Preparation of MS medium
5. Callus Initiation from Arachis hypogeal L.
6. Multiple shoot proliferation in Citrus nodal segment
7. Callus differentiation and organogenesis
8. Apical meristem culture to raise virus free plants.
9. Digestion of DNA using restriction enzymes and analysis by agarose gel electrophoresis
10. Ligation of DNA fragments
11. Isolation of plasmid DNA.
