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LABORATORY VI BIOPROCESS ENGINEERING AND TECHNOLOGY
The objectives of this laboratory course are to provide hands-on training to students in upstream and downstream unit operations.
1. Basic Microbiology techniques
a) Scale up from frozen vial to agar plate to shake flask culture.
b) Instrumentation: Microplate reader, spectrophotometer, microscopy.
c) Isolation of microorganisms from soil samples.
2. Experimental set-up
a) Assembly of bioreactor and sterilization.
b) Growth kinetics.
c) Substrate and product inhibitions.
d) Measurement of residual substrates.
3. Data Analysis
a) Introduction to Metabolic Flux Analysis (MFA).
4. Fermentation
a) Batch.
b) Fed-batch.
c) Continuous.
5. Unit operations
a) Microfiltrations: Separation of cells from broth.
b) Bioseparations: Various chromatographic techniques and extractions.
6. Bioanalytics
a) Analytical techniques like HPLC, FPLC, GC, GC-MS etc. for measurement
of amounts of products/substrates
