LABORATORY VIII

Paper Code: 
DMBL 704
Credits: 
02
Contact Hours: 
60
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course the learner will:

CO 74: Understand and relate to various genetic engineering techniques.

CO 75:  Use and illustrate various gene transfer techniques like conjugation and transformation

CO 76: Plan to prepare competent cells

CO 77: Pivot after understanding the principle underlying Gel electrophoresis and SDS page

CO 78: Appraise knowledge to isolate Plasmid DNA from bacterial cell and its digestion

CO 79: Design, modify and apply the working of PCR to research.

Discussion, Tutorials, Demonstration, Effective questions, Simulation, Giving tasks, Practical

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

CONTENTS

  1. Agarose gel electrophoresis
  2. Gene Transfer-Conjugation
  3. Transformation of E.coli
  4. Preparation of competent cells
  5. Bacteriophage Plaque Assay for Phage Titer
  6. Isolation of plasmid DNA from bacterial cell.
  7. Restriction Digestion
  8. Demonstration of PCR
  9. Demonstration of SDS-PAGE

 

Academic Year: