MICROBIAL GENETICS

Paper Code: 
MBL 221
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

       Course Outcomes

Learning and teaching strategies

Assessment Strategies

  
 

On completion of this course the learner will:

CO 42: Evaluate the basic mechanism of gene mutations

CO 43: Examine various bacterial gene transfer mechanisms

CO 44: Interpret the role of plasmid and transposons in genetic analysis

CO 45: Assess the life cycles of various bacteriophages

CO 46: Summarize the role of microbial genetics in vaccine designing.

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

  

 

10.00
Unit I: 
Introduction to gene and mutagenesis

·         Gene as UNIT of mutation and recombination. Molecular nature of mutations.

·         Physical and chemical mutagens.

·         Spontaneous mutations- random and non-adaptive mutation, mutation rates, origin.

Genetic analysis of mutants.

18.00
Unit II: 
Bacterial genetics
  • Gene transfer mechanisms-
  • Transformation- molecular mechanism, mapping and other uses of transformation, Transduction- generalized transduction, co transduction and linkage, mapping by co transduction, specialized transduction, specialized transducing phage as a cloning
  • vehicle.
  • Bacterial conjugation- insertion of F in E. coli, Hfr transfer, recombination in recipient cells. Chromosome transfer in other bacteria.

 

17.00
Unit III: 
Plasmids and Transposons
  • Plasmids- types and properties, F-factors description. Colicins and col factors. Plasmids as vectors for genetic cloning. Plasmid replication.
  • Transposons- types, genetic phenomena mediated by transposons in bacteria.
  • Use of plasmid and tranposons in genetic analysis.
15.00
Unit IV: 
Bacteriophages

Bacteriophages, Lytic phages-T7 and T4 Lysogenic phages Lambda phage, and P1, M13 and φ X174 life cycles, Phage MU and their uses in microbial genetics

15.00
Unit V: 
Role of microbial genetics in vaccine designing

Microbial genetics and design of vaccines. BCG and design of vaccine for TB and leprosy. DNA vaccines, design and advantages. 

ESSENTIAL READINGS: 
  • Microbiology-an introduction, 13th edition, G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020
  • Prescott’s Microbiology, 11th edition, J Willey, K Sandman, D Wood, Mc Graw Hill Companies, 2019
  • General microbiology, 7th edition, H S Schlegel, Cambridge University Press, 1995
  • Microbiology: principles and explorations, 9th edition, J G Black, L J Black, John Wiley and Sons, Inc, 2015
  • Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2006.
  • Concepts of Genetics, 11th edition, W S Klug, M R Cummings, C A Spencer, M A Palladino, Case Pearson Education, Inc., 2019
  • Lewin’s Genes X, 10th edition, J E Krebs, E Goldstein, S T Kilpatrick, Jones & Barlett Publishers, 2009
  • Cell and Molecular Biology, 8th edition, Gerald Karp, John Wiley and Sons. 2018.
  • Biochemistry and Molecular Biology, 4rd edition, Elliott W H and Elliot D C, Oxford University Press, 2009.
  • Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 2008.
REFERENCES: 

SUGGESTED READINGS:

  • Brock Biology of Microorganisms, 14th edition, M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • General Microbiology, 5th edition, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Fundamentals of microbiology, 11th edition, J C. Pommerville, Jones & Barlett Publishers, 2017
  • Foundation in Microbiology, 11th edition, K P Talaro and A Talaro, Mc Graw Hill, 2021
  • Brock Biology of Microorganisms, 14th edition M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • Essentials of Molecular Biology, 4rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 2015.
  • Instant Notes, Molecular Biology, 4rd edition, P G Turner, Mc Lennan, A D  Bates, and M R H White, Bios Scientific Publishers. 2016.
  • Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons Publications, 2009.

 

e RESOURCES:

 

JOURNALS:

 

Academic Year: 
2022-2023
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