MICROBIAL PHYSIOLOGY

Paper Code: 
MBL 502
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to understand –

 

  1. The nature of biological material and hierarchy of molecular organization of cells.
  2. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
  3. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

Course Outcomes (COs):

 

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 502

Microbial Physiology

CO 1 Acquaint with basics of metabolism and growthunder normal and stressed conditions.

CO 2 Study different types of transport mechanisms.

CO 3 Study photosynthesis and energy generating pathways.

CO 4  Learn about nitrogen fixation process.

CO 5 Understand chemolithotrophy.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

 

C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction
 
9.00
Unit I: 
Nutrient uptake

Biochemical properties of membrane, biochemical factors regulating the permeability, Nerst equation. Osmosis, Plasmolysis, passive & facilitated diffusion, active transport, secondary active transport, group translocation across membrane, role of ionophores.

8.00
Unit II: 
Photosynthesis

Photosynthetic microbes, oxygenic & anoxygenic photosynthesis, photophosphorylation, Calvin cycle.

11.00
Unit III: 
Respiratory Pathways

Glycolysis, Oxidative pentose phosphate pathway, Entner- Doudoroff pathway, fermentative pathways, Yeast fermentation, Lactic acid fermentation, Acetic acid fermentation, Krebs cycle, ETC, oxidative and substrate level phosphorylation.

9.00
Unit IV: 
Nitrogen Metabolism

Biological nitrogen fixation: process, components of nitrogenase system, oxygen sensitivity of nitrogenase enzyme, Nitrogen fixation in symbiotic and free living systems, Nitrate assimilation, transamination and deamination reactions.

8.00
Unit V: 
Chemolithotrophs

Hydrogen bacteria, nitrifying bacteria, sulfur bacteria, Iron bacteria, Methanogens and Methylotrophs.

ESSENTIAL READINGS: 

1.      Biochemistry, 5th edition L Stryer, W H Freeman and Co., New York, 2001

2.      General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999

3.      Lehninger Principles of Biochemistry, 3rd edition, D L Nelson and M M Cox Worth Publications Inc. CBS Publication New Delhi, 2000

REFERENCES: 
  1. An Introduction to Nitrogen fixation, J R Gallon and A E Chaplin, Cassell Education Ltd, 1987
  2. Biochemistry, 23rd edition, Harper, Appleton and Lange Prentice Hall, 1993
  3. Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
  4. Microbial Physiology and Metabolism, D R Caldwell, Brown Publishers, 1995
  5. Microbial Physiology, 4th edition, A G Moat and J W Foster, John Wiley and Sons, Inc., 2002
  6. Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006

 

Academic Year: 
2020-2021
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