Microbial Physiology

Paper Code: 
MBL 502
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to understand –

 

1.

he nature of biological material and hierarchy of molecular organization of cells.

  1. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
  2. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

 

Course Outcomes (COs):

 

 

Course

Learning    outcome                  (at course level)

Learning                   and teaching strategies

Assess ment Strate

gies

Paper Code

Paper Title

 

 

CO 68

Approach         in

C        A

 

 

Acquaint    with    basics    of

teaching:

test,

 

 

metabolism                   and

Interactive

Semest

 

 

growthunder normal   and

Lectures,

er end

 

 

stressed conditions.

Discussion,

examin

 

 

CO 69

Tutorials,

ations,

 

 

Study different   types   of

Reading

Quiz,

 

 

transport mechanisms.

assignments,

Solving

 

MBL 502

Microbia l Physiolo gy

CO 70

Study photosynthesis and energy                         generating pathways.

CO 71

presentations

 

Learning

activities for the students:

proble ms     in tutorial

s, Assign

 

 

Learn      about       nitrogen

Self-learning

ments,

 

 

fixation process.

assignments,

Classro

 

 

CO 72

Effective

om

 

 

Understand

questions,

interact

 

 

chemolithotrophy.

Seminar

ion

 

 

 

presentation,

 

 

 

 

Giving tasks.

 

 

9.00
Unit I: 
Nutrient uptake
  • Biochemical properties of membrane, biochemical factors regulating the permeability, Nerst equation. Osmosis, Plasmolysis, passive & facilitated diffusion, active transport, secondary active transport, group translocation across membrane, role of ionophores.

 

8.00
Unit II: 
Photosynthesis
  • Photosynthetic microbes, oxygenic & anoxygenic photosynthesis, photophosphorylation, Calvin cycle.

 

11.00
Unit III: 
Respiratory Pathways
  • Glycolysis, Oxidative pentose phosphate pathway, Entner- Doudoroff pathway, fermentative pathways, Yeast fermentation, Lactic acid fermentation, Acetic acid fermentation, Krebs cycle, ETC, oxidative and substrate level phosphorylation.

 

 

9.00
Unit IV: 
Nitrogen Metabolism
  • Biological nitrogen fixation: process, components of nitrogenase system, oxygen sensitivity of nitrogenase enzyme, Nitrogen fixation in symbiotic and free living systems, Nitrate assimilation, transamination and deamination reactions.

 

8.00
Unit V: 
Chemolithotrophs
  • Hydrogen bacteria, nitrifying bacteria, sulfur bacteria, Iron bacteria, Methanogens and Methylotrophs.

 

ESSENTIAL READINGS: 
  • Biochemistry, 5th edition L Stryer, W H Freeman and Co., New York, 2001
  • General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Lehninger Principles of Biochemistry, 3rd edition, D L Nelson and M M Cox Worth Publications Inc. CBS Publication New Delhi, 2000
  • An Introduction to Nitrogen fixation, J R Gallon and A E Chaplin, Cassell Education Ltd, 1987
  • Biochemistry, 23rd edition, Harper, Appleton and Lange Prentice Hall, 1993
  • Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
  • Microbial Physiology and Metabolism, D R Caldwell, Brown Publishers, 1995
  • Microbial Physiology, 4th edition, A G Moat and J W Foster, John Wiley and Sons, Inc., 2002
  • Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006

 

Academic Year: 
2021-2022
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