MICROBIAL PHYSIOLOGY

Paper Code: 
MBL 502
Credits: 
03
Contact Hours: 
45
Objective: 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO 64: Assess the properties of cell membrane and mechanisms of nutrient uptake

CO 65: Examine the process of photosynthesis

CO 66: Interpret the mechanism of different respiratory pathways

CO 67: Analyze the process of nitrogen metabolism

CO 68: Examine the process of chemolithotrophy

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

9.00
Unit I: 
Nutrient uptake
  • Biochemical properties of membrane, biochemical factors regulating the permeability, Nerst equation. Osmosis, Plasmolysis, passive & facilitated diffusion, active transport, secondary active transport, group translocation across membrane, role of ionophores.

 

8.00
Unit II: 
Photosynthesis
  • Photosynthetic microbes, oxygenic &anoxygenic photosynthesis, photophosphorylation, Calvin cycle.

 

11.00
Unit III: 
Respiratory Pathways
  • Glycolysis, Oxidative pentose phosphate pathway, Entner- Doudoroff pathway, fermentative pathways, Yeast fermentation, Lactic acid fermentation, Acetic acid fermentation, Krebs cycle, ETC, oxidative and substrate level phosphorylation.

 

9.00
Unit IV: 
Nitrogen Metabolism

Biological nitrogen fixation: process, components of nitrogenase system, oxygen sensitivity of nitrogenase enzyme, Nitrogen fixation in symbiotic and free living systems, Nitrate assimilation, transamination and deamination reactions.

8.00
Unit V: 
Chemolithotrophs
  • Hydrogen bacteria, nitrifying bacteria, sulfur bacteria, Iron bacteria, Methanogens and Methylotrophs.

 

ESSENTIAL READINGS: 
  • Microbiology ,5th edition, M J Pelczar, E C S Chan, N R Kreig, Tata Mc Graw Publication, 2019
  • Microbiology-a Laboratory Manual, 10th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2014
  • Microbiology-an introduction, 13th edition, G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020
  • Prescott’s Microbiology, 11th edition, J Willey, K Sandman, D Wood,  Mc Graw Hill Companies, 2019
  • Microbial Physiology and Metabolism, 2nd edition, D R Caldwell, Brown Publishers, 1999
  • Microbial Physiology, 4th edition, A G Moat and J W Foster, John Wiley and Sons, Inc., 2011
  • Biological Nitrogen Fixation, 1st edition, F J de Bruijn, Wiley- Blackwell Publishers, 2015

 

REFERENCES: 

SUGGESTED READINGS:

  • Advances in Microbial Physiology, 1st edition, R K Poole, Academic Press, 2006
  • Brock Biology of Microorganisms, 14th edition, M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • General Microbiology, 5th edition, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
  • Fundamentals of microbiology, 11th edition, J C. Pommerville, Jones &Barlett Publishers, 2017

e RESOURCES:

JOURNALS:

  • Frontiers in Microbiology
  • Journal of & Letters in Applied Microbiology
  • Microbiology and Molecular Biology Reviews
  • BMC Microbiology
  • FEMS Microbiology Letters
  • PLOS ONE
  • Nature

 

Academic Year: 
2022-2023
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