Microbial Physiology

Paper Code: 
MBL 502
Credits: 
3
Contact Hours: 
45
Objective: 

 

·      Study different types of transport mechanisms.

·       Study photosynthesis and energy generating pathways.

·       Learn about nitrogen fixation process.

·       Understand chemolithotrophy.

9.00
Unit I: 
Nutrient uptake

 

                                                                                 

Biochemical properties of membrane, biochemical factors regulating the permeability, Nerst equation. Osmosis, Plasmolysis, passive & facilitated diffusion, active transport, secondary active transport, group translocation across membrane, role of ionophores.

 

 

 

9.00
Unit II: 
Photosynthesis

                                                  

Photosynthetic microbes, oxygenic & anoxygenic photosynthesis, photophosphorylation, Calvin cycle.

 

 

 

 

11.00
Unit III: 
Respiratory Pathways

                                              

Glycolysis, Oxidative pentose phosphate pathway, Entner- Doudoroff pathway, fermentative pathways, Yeast fermentation, Lactic acid fermentation, Acetic acid fermentation, Krebs cycle, ETC, oxidative and substrate level phosphorylation.

9.00
Unit IV: 
Nitrogen Metabolism

                                                                  

Biological nitrogen fixation: process, components of nitrogenase system, oxygen sensitivity of nitrogenase enzyme, Nitrogen fixation in symbiotic and free living systems, Nitrate assimilation, transamination and deamination reactions.

 

 

 

9.00
Unit V: 
Chemolithotrophs

                                                                                 

Hydrogen bacteria, nitrifying bacteria, sulfur bacteria, Iron bacteria, Methanogens and Methylotrophs.

 

ESSENTIAL READINGS: 

1.     Biochemistry, 5th edition L Stryer, W H Freeman and Co., New York, 2001

2.     General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999

3.     Lehninger Principles of Biochemistry, 3rd edition, D L Nelson and M M Cox Worth Publications Inc. CBS Publication New Delhi, 2000

 

 

 

 

REFERENCES: 

An Introduction to Nitrogen fixation, J R Gallon and A E Chaplin, Cassell Education Ltd, 1987

  1. Biochemistry, 23rd edition, Harper, Appleton and Lange Prentice Hall, 1993
  2. Biology of Microorganisms, 10th edition, T D Brock, M T Madigan, Pearson Education, Inc., 2003
  3. Microbial Physiology and Metabolism, D R Caldwell, Brown Publishers, 1995
  4. Microbial Physiology, 4th edition, A G Moat and J W Foster, John Wiley and Sons, Inc., 2002
  5. Microbiology-a Laboratory Manual, 6th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson Education, Inc., 2006
Academic Year: 
2015-2016
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