MICROBIOLOGY AND BIOPROCESS TECHNOLOGY

Paper Code:

CBTE 301

Credits:

Contact Hours:

Objective:

Course Outcomes	Learning and teaching strategies	Assessment Strategies
Upon completion of the course the learner will: CO21: Apply the knowledge of aseptic concepts to laboratory/practical work CO22: Identify the different types of microbes CO23: Analyze the effect of different factors on microbial growth CO24: Correlate designing and working of various fermenters CO25: Analyze medium formulation, inoculum preparation and sterilization CO26: Formulate recovery operations together with the fundamental principles for basic methods in production technique for bio-based products.	Class lectures Seminars Tutorials Group discussions and Workshops Question preparation	Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO21: Apply the knowledge of aseptic concepts to laboratory/practical work

CO22: Identify the different types of microbes

CO23: Analyze the effect of different factors on microbial growth

CO24: Correlate designing and working of various fermenters

CO25: Analyze medium formulation, inoculum preparation and sterilization

CO26: Formulate recovery operations together with the fundamental principles for basic methods in production technique for bio-based products.

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

12.00

Unit I:

History of Microbiology and Sterilization

Development of Microbiology (contribution of pioneers).

Concept of sterilization, methods of sterilization-dry heat, wet heat, radiation, filtration and chemicals

14.00

Unit II:

Introduction to Microbial World

Introduction to different types of microbes: bacteria, viruses, algae, fungi and protozoa. Structure of bacterial cell- cell membrane, cell wall, flagella and motility, cell inclusion bodies, Sporulation in bacteria.

Nutritional classification of Microorganisms.

10.00

Unit III:

Growth of Microorganisms

Microbial growth, growth curve, factors affecting microbial growth (pH, temperature, oxygen and water availability).

12.00

Unit IV:

Bioreactors and Fermentation Process

Bioreactors: Basic Design and components Types of microbial culture– Batch, Fed batch and Continuous culture. Principles of upstream processing – Media preparation, Inocula development and sterilization.

12.00

Unit V:

Principles and Operation of Downstream Processing

Cell disruption, filtration, centrifugation, liquid-liquid extraction, solvent extraction (distillation), preparative ion exchange chromatography, drying.

ESSENTIAL READINGS:

Microbiology, 5^th edition M J Pelcer, E C S Chan, N R Kreig, Tata Mc Graw Publication,2006

● Microbiology-a Laboratory Manual, 6^th edition, J G Cappuccino and N Sherman, Addison Wesley, Pearson education, Inc., 2006

● Microbiology-an introduction, 13^th edition, G.J. Tortora, B.R. Funke, C.L. Case Pearson Education, Inc., 2020

● Principles of Fermentation Technology by P. Stanbury, A. Whitaker, S. Hall. 3^rd edition. Butterworth-Heinemann. 2016.

● Fermentation Microbiology and Biotechnology edited by E.M.T. El-Mansi, C.F. Bryce, A.L. Demain, A.R. Allman. 3^rd edition. CRC Press. 2012

Bioprocess Engineering: Basic Concepts by M. L. Shuler, F. Kargi, 2 nd edition. Pearson Education India. 2015

REFERENCES:

SUGGESTED READINGS:

● Biology of Microorganisms, 10^th edition, T D Brock, M T Madigan, Pearson education, Inc., 2003

● Prescott’s Microbiology, 11^th edition, J Willey, K Sandman, D Wood, Mc Graw Hill Companies, 2019

● Bioprocess Engineering (2^nd edition) by Michael L.Schuler and F.Kargi .Prentice Hall of India.2002.

● Process Biotehnology Fundamentals (2^nd edition by S.N. Mukhopadhyaya. Vivi Books Pvt. Ltd.2004

● Modern Industrial Microbiology & Biotechnology by N. Okafor. 1^st edition. CRC Press, USA. 2007.

● Microbial Biotechnology: Fundamentals of Applied Microbiology by A.N. Glazer, H. Nikaido. 2 nd edition. Cambridge University Press. 2007

● Process Biotehnology Fundamentals (2^nd edition by S.N. Mukhopadhyaya. Vivi Books Pvt. Ltd.2004.

● Manual of Industrial Microbiology and Biotechnology (2^nd edition). Edited by A.L. Demain and J. E Davies, Panima Publications 2004

● Roger G. Harrison, Paul W. Todd, Scott R. Rudge, Demetri P. Petrides, Bioseparations Science and Engineering, 2^nd Ed., Oxford University Press, 2003.

● Pauline M. Doran, Bioprocess Engineering Principles, 2^nd Ed., Academic Press, 2012

● James Bailey, David Ollis, Biochemical Engineering Fundamentals, 2^nd Ed., McGraw Hill Education, 2017

eRESOURCES:

● https://onlinelibrary.wiley.com/doi/book/10.1002/9781119237860

● http://www.springerlink.com/content/jtl6g6/?p=480efe17b23542a79b20f0637fba5f9a&pi=1

● https://vufind.carli.illinois.edu/vf-iit/Record/iit_781305

● https://doi.org/10.1128/9781555819972.ch5

JOURNALS:

• Journal of Biochemical Engineering & Bioprocess Technology

• Advances in Bioprocess Technology(Elsevier)

Biotechnology and Bioprocess Engineering(Springer)

Academic Year: