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Molecular Biology
To enable the student to: • Understand the Molecular basis of life. • Flow of genetic onformation. • Regulation of gene expression.
Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization
DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases
Prokaryotic & Eukaryotic transcription (Initiation ,Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability
Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.
Molecular mechanism of antisense molecules-Inhibition of splicing, polyadenylation, translation. disruption of RNA structure & capping, Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb & p53 tumor suppressor proteins.
1. Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2000. 2. Introduction to Practical Molecular Biology, P. D. Dabre, John Wiley & Sojns Ltd.,Yourk,1988. 3. Genomes. Brown T.A: John Wiley and sons(ASIA) PTE Ltd1999 4. Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994. 5. Molecular Biology of the Gene. (5th Edition) Watson, Baker, Bell, Gann, Levin, Losick: Pearson Education.2003. 6. Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994. 7. Essentials of Molecular Biology. Malacinski, G M, Freifelder D: Jones & Bartlett Publishers Inc. (1998) 8. From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003
9. Genectics – A molecular approach by Russell, Peter. J, 2003 10. Gene IX (9th Edition) Benjamin Lewin. Oxford University Press.U.K., 2007 11. Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.
