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Molecular Biology
Course Objectives:
This course will enable the students to –
- understand the central dogma of molecular biology.
- understand process of prokaryotic and eukaryotic replication.
- know machinery involved in Prokaryotic and eukaryotic transcription.
- get a detailed insight of mechanism and regulation of prokaryotic and eukaryotic translation process.
- study the molecular mechanisms of antisense and ribozyme technology.
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BTE/MBL 222
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Molecular Biology
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Upon completion of the course students will be able to: CO 33: Assess prokaryotic and eukaryotic gene structure and function CO 34: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication CO 35: Assess gene regulation in both prokaryotes and eukaryotes CO 36: Evaluate translation mechanism in prokaryotes and eukaryotes CO 37: Evaluate molecular mechanisms of Antisense and ribozyme technology |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments Learning activities for the students: Self learning assignments, Effective questions, Giving tasks |
Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation |
Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization
DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.
Prokaryotic & Eukaryotic transcription (Initiation ,Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability.
Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.
Molecular mechanism of antisense molecules-Inhibition of splicing, polyadenylation, translation. disruption of RNA structure & capping, Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb& p53 tumor suppressor proteins.
