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Molecular Biology
Course Objectives:
This course will enable the students to –
- Understand the Molecular basis of life.
- Flow of genetic information.
- Regulation of gene expression
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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MBL 222
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Molecular Biology
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Upon completion of the course students will be able to CO 35: Assess prokaryotic and eukaryotic gene structure and function CO 36: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication CO 37: Assess gene regulation in both prokaryotes and eukaryotes CO 38: Assess translation mechanism in prokaryotes and eukaryotes CO 39: Analyze molecular mechanisms of Antisense and ribozyme technology |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the students: Self learning assignments, Effective questions, Giving tasks |
Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation |
- Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization
DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.
Prokaryotic & Eukaryotic transcription (Initiation ,Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability.
- Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.
Molecular mechanism of antisense molecules-Inhibition of splicing, polyadenylation, translation. disruption of RNA structure & capping, Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb& p53 tumor suppressor proteins.
BOOKS RECOMMENDED:
- Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2000.
- Introduction to Practical Molecular Biology, P. D. Dabre, John Wiley &Sojns Ltd.,Yourk,1988.
- Genomes. Brown T.A: John Wiley and sons(ASIA) PTE Ltd1999
- Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
- Molecular Biology of the Gene. (5th Edition) Watson, Baker, Bell, Gann, Levin, Losick: Pearson Education.2003.
- Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books, USA, 1994.
- Essentials of Molecular Biology. Malacinski, G M, Freifelder D: Jones & Bartlett Publishers Inc. (1998)
- From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003
- Genectics – A molecular approach by Russell, Peter. J, 2003
- Gene IX (9th Edition) Benjamin Lewin. Oxford University Press.U.K., 2007
- Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.
