MOLECULAR BIOLOGY

Paper Code: 
BTE 222
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

 

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

 
 

Upon completion of the course the learner will:

CO 49: Illustrate prokaryotic and eukaryotic gene structure and function

CO 50: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication

CO 51: Correlate gene regulation in prokaryotes and eukaryotes

CO 52: Analyze translation mechanism in prokaryotes and eukaryotes

CO 53: Assess molecular mechanisms of Antisense and ribozyme technology

Interactive Lectures,

Discussion,

Tutorials,

Demonstrations,

Assignments

Reading Journals

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

  
 

 

 

12.00
Unit I: 
Prokaryotic and Eukaryotic gene structure and function
  • Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization

 

15.00
Unit II: 
Replication in Prokaryotes and Eukaryotes
  • DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins  involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.

 

 

15.00
Unit III: 
Transcription in Prokaryotes and Eukaryotes
  • Prokaryotic & Eukaryotic transcription (Initiation, Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability.

 

15.00
Unit IV: 
Machinery involved in Prokaryotic and Eukaryotic Translation
  • Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.

 

18.00
Unit V: 
Antisense and Ribozyme Technology
  • Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb& p53 tumor suppressor proteins
ESSENTIAL READINGS: 

ESSENTIAL READINGS:

  • Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2012.
  • Genomes. Brown T.A: Published by Garland Science Publishing, a member of the Taylor & Francis Group, 2002
  • Molecular Biology of the Cell (6th Edition) Bruce Albert, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter, 2014
  • Molecular Biology of the Gene (7th Edition) Watson, Baker, Bell, Gann, Levin, Losick:  Pearson Education.2017.
  • Molecular Cell Biology (9th Edition) J. Darnell, H. Lodish and D.Baltimore, Macmillan learning, 2021.
  • Freifelder’s Essentials of Molecular Biology.  Malacinski, G M, Jones & Bartlett Publishers Inc. (2015)

SUGGESTED READINGS:

  • From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003
  • iGenetics – A molecular approach by Russell, Peter. J, 2016.
  • Gene XI Benjamin Lewin. Oxford University Press.U.K., 2013.
  • Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.

 

REFERENCES: 

e RESOURCES

     JOURNAL:

 

 

Academic Year: 
2022-2023
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