MOLECULAR BIOLOGY

Paper Code: 
BTE 302
Credits: 
3
Contact Hours: 
45
Objective: 

Upon completion of the course the learner will:

CO37: Illustrate prokaryotic and eukaryotic gene structure and function

CO38: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication

CO39: Correlate gene regulation in prokaryotes and eukaryotes

CO 40: Analyze translation mechanism in prokaryotes and eukaryotes

CO41: understand the regulation of gene expression
 

 

8.00
Unit I: 
DNA: The genetic material
  • DNA and Genome: The primary structure of DNA, packaging of DNA, Gene in molecular terms, physical and chemical nature of nucleic acid,types of DNA (A, B, Z).

 

 

12.00
Unit II: 
DNA replication and recombination
  • DNA replication in prokaryotes and eukaryotes, enzymes involved in replication, DNA repair mechanism- mismatch repair, base excision repair, Nucleotide excision repair photoreactivation, Recombination and repair (Holiday model)

 

12.00
Unit III: 
Transcription and Post-transcriptional modifications
  • Transcription in prokaryotes and eukaryotes, mRNA structure (coding and non coding RNA; miRNA, siRNA and hnRNA), Ribozyme, Posttranscriptional modifications. 

 

 

8.00
Unit IV: 
Translation and Post-translational modifications
  • Genetic code, Prokaryotic and eukaryotic translation, Post translational modification of proteins and its breakdown, inhibitors of protein synthesis.

 

 

8.00
Unit V: 
Regulation of gene expression
  • Regulation of gene expression in prokaryotes and eukaryotes at transcription and translation level, Operon concept-positive and negative regulation of lac operon, trp operon

 

 

ESSENTIAL READINGS: 
  • Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York,2012.
  • Genomes. Brown T.A: Published by Garland Science Publishing, a member of the Taylor & Francis Group, 2002
  • Molecular Biology of the Cell (6th Edition) Bruce Albert, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter, 2014
  • Molecular Biology of the Gene (7th Edition) Watson, Baker, Bell, Gann, Levin, Losick:  Pearson Education.2017.
  • Molecular Cell Biology (9th Edition) J. Darnell, H. Lodish and D.Baltimore, Macmillan learning, 2021.
  • Freifelder’s Essentials of Molecular Biology.  Malacinski, G M, Jones & Bartlett Publishers Inc. (2015)

 

REFERENCES: 

Suggested Reading:

  • From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003
  • iGenetics – A molecular approach by Russell, Peter. J, 2016.
  • Gene XI Benjamin Lewin. Oxford University Press.U.K., 2013.
  • Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.

 

e RESOURCES:

 

Journal:

 

Academic Year: 
2022-2023
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