MOLECULAR BIOLOGY

Paper Code:

MBL 222

Credits:

Contact Hours:

Objective:

Course Outcomes (COs):

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO 47: Illustrate prokaryotic and eukaryotic gene structure and function

CO 48: Compare and contrast the mechanisms of bacterial and eukaryotic DNA replication

CO 49: Correlate gene regulation in prokaryotes and eukaryotes

CO 50: Analyze translation mechanism in prokaryotes and eukaryotes

CO 51: Assess molecular mechanisms of Antisense and ribozyme technology

Interactive Lectures,

Discussion,

Tutorials,

Demonstrations,

Assignments

Reading Journals

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

12.00

Unit I:

Prokaryotic and Eukaryotic gene structure and function

Structure and properties of nucleic acids, Central dogma of molecular biology, Prokaryotic gene structure and organization, Eukaryotic genome structure and chromosome organization

15.00

Unit II:

Replication in Prokaryotes and Eukaryotes.

DNA Replication in prokaryotes & eukaryotes, enzymes and accessory proteins involved in DNA Replication, Recombination (Holliday model), FLP/ FRT and Cre/ Lox recombination, Rec A and other recombinases.

15.00

Unit III:

Transcription in Prokaryotes and Eukaryotes

Prokaryotic & Eukaryotic transcription (Initiation, Elongation & Termination), RNA Polymerase, General & Specific Transcription Factors, Regulatory elements & mechanism of transcription regulation, Post transcriptional gene silencing(PTGS), Modifications in RNA (5’ cap formation, Transcription termination, 3’end processing & Polyadenylation, Splicing, Editing), Nuclear export of mRNA, m-RNA stability.

15.00

Unit IV:

Machinery involved in Prokaryotic and Eukaryotic Translation

Prokaryotic and Eukaryotic Translation, the translation machinery, Mechanism of initiation, elongation, termination, Regulation of translation, Co & post translational modification of proteins, Localization of proteins, synthesis of secretory & membrane proteins, Import into nucleus, mitochondria, chloroplast & peroxisomes, receptor mediated endocytosis.

18.00

Unit V:

Antisense and Ribozyme Technology.

Biochemistry of ribozymes- hammerhead, hairpin, other ribozymes, strategies for designing ribozymes, Application of antisense & ribozyme technology, Viral & cellular oncogenes, tumor suppressor genes from humans, structure, function and Mechanism of action of Prb& p53 tumor suppressor proteins.

ESSENTIAL READINGS:

Molecular Cloning: a Laboratory Manual, J. Sambrook, E. F. Fritsch and I. Maniatis, Cold Spring harbor Laboratory Press, New York, 2012.
Genomes. Brown T.A: Published by Garland Science Publishing, a member of the Taylor & Francis Group, 2002
Molecular Biology of the Cell (6^th Edition) Bruce Albert, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter, 2014
Molecular Biology of the Gene (7^th Edition) Watson, Baker, Bell, Gann, Levin, Losick: Pearson Education.2017.
Molecular Cell Biology (9th Edition) J. Darnell, H. Lodish and D.Baltimore, Macmillan learning, 2021.
Freifelder’s Essentials of Molecular Biology. Malacinski, G M, Jones & Bartlett Publishers Inc. (2015)

REFERENCES:

SUGGESTED READING:

· From Genes to Clones by Winnacker, Panima Publishing Corporation, New Delhi/Bangalore, 2003

· Genetics – A molecular approach by Russell, Peter. J, 2016.

· Gene XI Benjamin Lewin. Oxford University Press.U.K., 2013.

· Molecular Biology and biotechnology. A comprehensive desk reference. R. A. Meyers (Editor) VCH Publishers, Inc., New York, 1995.

e Resources:

· https://open.umn.edu/opentextbooks/textbooks/244

· https://www.web-books.com/MoBio/Free/Chap3.htm

· https://www.web-books.com/MoBio/Free/Chap4.htm

· https://www.web-books.com/MoBio/Free/Chap5.htm

· https://www.web-books.com/MoBio/Free/Chap6.htm