MOLECULAR BIOLOGY

Paper Code: 
BTE 302
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

  • understand the basic processes involved in the flow of genetic information
  • study the processing of the protein synthesized
  • understand the regulation of gene expression
8.00
Unit I: 
DNA: The genetic material

DNA and Genome: The primary structure of DNA, packaging of DNA, Gene in molecular terms

 

10.00
Unit II: 
DNA replication and recombination

DNA replication in prokaryotes and eukaryotes, enzymes involved in replication. DNA repair mechanism- mismatch repair, base excision repair, photoreactivation, SOS response, Recombination and repair, Homologous recombination

12.00
Unit III: 
Transcription and Post-transcriptional modifications

Transcription in prokaryotes and eukaryotes. mRNA structure, mRNA synthesis. Ribozyme. Posttranscriptional modification. Regulation of gene expression in prokaryotes and eukaryotes.

 

12.00
Unit IV: 
Translation and Post-translational modifications

Prokaryotic and eukaryotic translation, Mechanism of initiation, elongation, termination. Post translational modification of proteins and breakdown.

4.00
Unit V: 
Regulation of gene expression

Regulation of gene expression in prokaryotes and eukaryote, Environmental factors responsible for regulation of gene expression, Developmental regulation of gene expression. 

ESSENTIAL READINGS: 
  1. Molecular Biology and Biotechnology, H.D. Kumar, 2nd revised version, Vikas Publishing House, 1996
  2. Principles of Genetics, 8th edition, Gardner, Simmons, Snustad, 2003
  3. Genomes by T.A. Brown, Bios Scientific Publishers Ltd., 1999
REFERENCES: 
  1. Cell and Molecular Biology- Concepts and Experiments, 4th edition, Gerald Karp, Willey International Edition, 2005
  2. Molecular Biology, Instant Notes, 2nd edition, P.C. Turner, A.G. Mc Lennan, A.D. Bates and M.R.H. White, Viva Books Pvt. Ltd., 2002
  3. Biochemistry and Molecular Biology- William H. Elliot, Daphne C. Elliot, Oxford University Press, 2005.
  4. Molecular Genetics, 2nd edition, An Introductory Narrative, Gunther S. Stent, Richard Calendar
  5. Essentials of Molecular Biology, 3rd edition by Malacinski and Freifelder, Jones and Bartlet Publishers, 1998
  6. Nucleic Acids by Shawn Doonan, University of East, London, 2004.
  7. Principles and Technique of Biochemistry and Molecular Biology, 6th edition, Keith Wilson and John Walker, Cambridge University Press, 2007.
Academic Year: 
2014-2015
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