Molecular Biology and Microbial Genetics

Paper Code: 
MBL 301
Credits: 
03
Contact Hours: 
45
Objective: 

 

  1. The nature of biological material and hierarchy of molecular organization of cells.
  2. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
  3. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

 

 

Course

Learning      outcome                     (at course level)

Learning and teaching

strategies

Assess ment Strate

gies

Paper Code

Paper Title

 

 

 

 

 

 

 

 

MBL 301

 

 

 

 

 

Molec ular Biolog y and Micro bial Genet ics

CO 33

Understand the Genetic constituents of bacteria with special emphasis on inheritance and mutations

CO 34

Understand Process of DNA replication                       transcription, translation

CO 35

Understand the mechanism of genetic transfers in microbes CO 36

Identification of                       genetic regulatory     mechanism                   and distinguishing                         different mechanism of gene regulation CO 37

Understand               molecular

mechanism of mutations and DNA repair

Approach          in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

C        A

test, Semest er end examin ations, Quiz, Solving proble ms     in tutorial s, Assign ments, Classro om interact ion

 

10.00
Unit I: 
DNA Structure and Replication

DNA as a genetic material, structure of DNA and RNA, Types of DNA (A, B, Z), DNA replication in prokaryotes and eukaryotes

8.00
Unit II: 
Transcription
  • Mechanism of Transcription in prokaryotes and eukaryotes, enzymes and transcription factors, Posttranscriptional modifications of eukaryotic mRNA.

 

10.00
Unit III: 
Translation and Regulation of Gene Expression
  • The genetic code, Translational machinery in eukaryotes and prokaryotes, inhibitors of protein synthesis
  • Regulation of Gene expression in prokaryotes (lac and trp operon)

 

9.00
Unit IV: 
Mutations and Mutant Selection
  • Molecular mechanism of mutation, forward and reverse mutation, transition, transversion, Spontaneous and induced mutations
  • Methods for mutant selection- direct selection, antibiotic selection, replica plating
  • Mechanism of DNA repair-photo reactivation, Nucleotide excision repair, mismatch repair

 

8.00
Unit V: 
Recombination in Bacteria
  • Transformation, transduction and conjugation
  • A brief idea of cloning vectors (plasmids, cosmids, bacteriophages).

 

ESSENTIAL READINGS: 
  • Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2005.
  • Gene VI, Lewin, B, New York, Oxford University Press, 2000.
  • Cell and Molecular Biology, 4th edition, Gerald Karp, John Wiley and Sons. 2005.
  • Biochemistry and Molecular Biology, 3rd edition, Elliott W H and Elliot D C, Oxford University Press, 2005.
  • Essentials of Molecular Biology, 3rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 1998.
  • Instant Notes, Molecular Biology. Turner P.G., Mc Lennan, Bates A D, and White M R H, 2nd edition. Bios Scientific Publishers. 2002.
  • Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons. 1987.
  • Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 1994.

 

Academic Year: 
2021-2022
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