MOLECULAR BIOLOGY AND MICROBIAL GENETICS

Paper Code: 
MBL 301
Credits: 
03
Contact Hours: 
45
Objective: 

TThis paper will enable students to understand –

 

  1. The nature of biological material and hierarchy of molecular organization of cells.
  2. The thermodynamic principles underlying biochemical reactions and the function of macromolecules.
  3. The students will learn the concept of enzyme catalysis, reaction mechanism and regulation of enzyme activity

Course Outcomes (COs):

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 301

Molecular Biology and Microbial Genetics

CO 1UUnderstand the Genetic constituents of bacteria with special emphasis on inheritance and mutations

CO 2UUnderstand Process of DNA replication transcription, translation

CO 3UUnderstand the mechanism of genetic transfers in microbes

CO 4iIdentification of genetic regulatory mechanism and distinguishing different mechanism of gene regulation

CO 5UUnderstand molecular mechanism of mutations and DNA repair

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students:

Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

 

C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction

 

10.00
Unit I: 
DNA Structure and Replication

                                                                            

DNA as a genetic material, structure of DNA and RNA, Types of DNA (A, B, Z), DNA replication in prokaryotes and eukaryotes.

8.00
Unit II: 
Transcription

                                                                                         

Mechanism of Transcription in prokaryotes and eukaryotes, enzymes and transcription factors, Posttranscriptional modifications of eukaryotic mRNA.

 

10.00
Unit III: 
Translation and Regulation of Gene Expression

                       

The genetic code, Translational machinery in eukaryotes and prokaryotes, inhibitors of protein synthesis

Regulation of Gene expression in prokaryotes (lac and trp operon)

9.00
Unit IV: 
Mutations and Mutant Selection

Molecular mechanism of mutation, forward and reverse mutation, transition, transversion, Spontaneous and induced mutations

Methods for mutant selection- direct selection, antibiotic selection, replica plating

Mechanism of DNA repair-photo reactivation, Nucleotide excision repair, mismatch repair

 

8.00
Unit V: 
Recombination in Bacteria

                                                        

Transformation, transduction and conjugation

A brief idea of cloning vectors (plasmids, cosmids, bacteriophages).

 

 

ESSENTIAL READINGS: 
  1. Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2005.
  2. Gene VI, Lewin, B, New York, Oxford University Press, 2000.
  3. Cell and Molecular Biology, 4th edition, Gerald Karp, John Wiley and Sons. 2005.

 

REFERENCES: 
  1. Biochemistry and Molecular Biology, 3rd edition, Elliott W H and Elliot D C, Oxford University Press, 2005.
  2. Essentials of Molecular Biology, 3rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 1998.
  3. Instant Notes, Molecular Biology. Turner P.G., Mc Lennan, Bates A D, and White M R H, 2nd edition. Bios Scientific Publishers. 2002.
  4. Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons. 1987.
  5. Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 1994.
Academic Year: 
2020-2021
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