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Paper III: Tools and Techniques (Advance Paper-I)
Paper Code:
LSC 143 D
Credits:
04
Contact Hours:
60
12.00
Unit I:
Spectroscopy
: Spectroscopy and Spectrometry, Types of Spectroscopy. Atomic Spectroscopy: Flame Emission Spectroscopy and X- Ray Spectroscopy Molecular Spectroscopy: IR Spectroscopy, UV Spectroscopy and Visible Spectroscopy Nuclear Spectroscopy: NMR Spectroscopy
12.00
Unit II:
Microscopy:
Microscopy: Magnifiers and microscopes, simple and compound microscopes. Types of microscopy – Optical Microscopy (Bright Field and Dark Field Microscopy, Phase contrast Microscopy, Fluorescence Microscopy, Confocal and Wide Field Deconvolution Microscopy ); Electron Microscopy (SEM, TEM).
12.00
Unit III:
Histology of Tissues:
Histology of Tissues: Selection of material, chemical fixation, fixatives, tissue dehydration using a graded solvent series of ethanol or acetone, rehydration, infiltration and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy (cryotome, cryostat and vibratome).
12.00
Unit IV:
Chromatography
Technique, types, applications and limitations.
Techniques based on bed shape- Column Chromatography, Paper Chromatography and Thin Layer Chromatography
Techniques based on physical state of mobile phase- Gas Chromatography, Liquid Chromatography and High Pressure Liquid Chromatography (HPLC).
Techniques based on separating mechanism- Ion Exchange Chromatography and Size Exclusion Chromatography
12.00
Unit V:
Microbiological techniques:
Microbiological techniques: Sterilization and disinfection, Simple and Complex Media, Preparation of media -solid and liquid media, nutrient agar slabs, slants and plates, Staining techniques, simple and differential staining, Instrumentation, Inoculation, Pure culture techniques: streak plate, pour plate and spread plate method.
ESSENTIAL READINGS:
- A Biology Guide to Principles and Techniques of Practical Biochemistry. Wilson, K & Goulding, KH. ELBS edition.
- Introduction to Instrumental Analysis. Robert Brown. Mc Graw Hill Internatiuonal Edition.
- Introduction to Practical Molecular Biology. Dabre, PG. John Wiley & Sons Ltd.
- Kuby Immunology (fourth edition). Golds, RA. Thomas J. Kintz, Barbara, A. Osborne, Freeman & Co., New York.
- Microbiological Applications: A Laboratory Manual in General Microbiology. Benson, HJ. WCG; WnC Brown Publishers.
- Microbiology, A Laboratory Manual. Cappuccino, JG and Sherman, N. Addison Wesley.
- Molecular and Cellular Methods in Biology & Medicine. 1995. Win, K, Kim & Cseke. CRC Press, Florida
- Molecular Cloning: A Laboratory Manual. Sambrook,J Fritsch, EF & Maniatis, T. Cold Spring Harbor Laboratory Press, New York.
Academic Year:
