Paper IV: Microbiology (Advance Paper-II)

Paper Code: 
LSC 144 A
Credits: 
04
Contact Hours: 
60
12.00
Unit I: 
TAXONOMY
Microbial evolution, systematic and taxonomy-Evolution of earth and earliest life forms; primitive organisms, their metabolic strategies and molecular coding; approaches to bacterial taxonomy, DNA-DNA and DNA-RNA hybridization, G+C content determination, ribotyping, Ribosomal RNA sequencing; Characteristics of primary domains, Taxonomy, Nomenclature and Bergey's Manual. Culture collection, maintenance and preservation of microorganisms.

 

12.00
Unit II: 
EXTREMOPHILES
 

Hyperthermophiles, halophiles halophilic thermophiles.

Heat, cold and deep sea adaptations. Exploitation of extremophiles in medicine, agriculture and industries

 

12.00
Unit III: 
DETECTION, ENUMERATION AND IDENTIFICATION OF MICROBES
 

Environmental sample collection and processing. Microscopic techniques: Fluororescence (direct count, fluorescent  immunolabelling, FISH . Electro Microscopy: Scanning Transmission, elemental analysis), Confocal Laser scanning  microscopy, Scanning Probe microscopy ( atomic force microscopy), flow cytometry

Culture methods: isolation and enumeration of bacteria, media for bacteria, culture methods of algae, fungi and viruses.

Physiological methods: carbon respiration, incorporation of radiolabelled traces  into cellular macromolecules, adenylate energy charges, enzyme assays stable isotope probing, functional genomics and proteomics –based approaches

 

12.00
Unit IV: 
AQUATIC MICROBIOLOGY
 

Microbial habitats in aquatic environments (planktonic, Benthic, microbial mats, biofilms).Aquatic environments; (fresh water, brackish water, marine water, subterranean).

 

12.00
Unit V: 
SOIL MICROBIOLOGY
 

Micro flora of various soil types, Culture methods for enumeration of soil microorganisms, physiochemical characteristics, soil as a microbial environment microorganism in surface soil, shallow & in the deep subsurface.

 

ESSENTIAL READINGS: 
  • Molecular Genetics of Bacteria, Larry Shyder and Wendy Champness, ASM Press,1997.
  • Introduction to Geomicrobiology, Kurt Konhauser, Blackwell Publishing,2007.
  • Environmental Biotechnology: Basic Concepts and Applications, Indu Shekhar Thakur, IK International, 2006.
  • Medical Microbiology and Immunology: Examination and Board Review
  • Warren Levinson and Ernst Jawetz, (7th edition), Mc Graw Hill, 2002.
  • Microbial Diversity: Current Prospective and Potential Applications, T. Satyanarayana, B.N. Johri, IK International, 2005.
Academic Year: 
2015-2016
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