PLANT BIOTECHNOLOGY

Paper Code: 
BTE 225
Credits: 
5
Contact Hours: 
75
Objective: 

Course Outcomes (COs):

              

                    Course Outcomes

Learning and teaching strategies

Assessment Strategies

  
 

On completion of this course the learner will:

CO 65:  Acquire knowledge of fundamentals of Plant Tissue culture

CO 66:  Acquire knowledge about basic in vitro propagation techniques & other techniques employed for plant improvement.

CO 67:   Acquaint with the genetic manipulations in Plant tissues and use of different markers in Plant Biotechnology.

CO 68:   Analyze various industrial applications of Plant Biotechnology.

CO 69:   Get acquainted with certification & legal

aspect of seed biology and to learn terminator

gene technology 

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation

  

 

13.00
Unit I: 
Introduction to plant tissue culture
  • Aseptic technique, Safety, Preparation and sterilization of apparatus & reagents, synthetic media, culture vessels used. Plant tissue culture: Concept of totipotency, dedifferentiation and redifferentiation.  Callus growth patterns/characteristics, organogenesis, hairy root culture. Somaclonal variations, somatic embryogenesis, synthetic seeds, anther and pollen culture, embryo culture and significance of haploid plants.

 

18.00
Unit II: 
Protoplast technology & micropropagation in plants
  • Isolation & fusion, somatic hybridization and cybrids. Production of virus free plants, Production of secondary metabolites, indexing for plant pathogens, Plant tissues culture and germplasm conservation. Material for Micropropagation, Advantages of Micropropagation, Types and Methods of Micropropagation and Applications of Micropropagation, Plant tissue culture techniques for crop improvement
20.00
Unit III: 
Plant as chemical and pharmaceutical factories
  • Techniques for production of transgenic plants resistant to herbicides, pathogens, pests and abiotic stresses (drought, salt & frost). PR Proteins, Nematode resistance, antifungal proteins, thionins, long shelf life of fruits & flowers, use of ACC synthase, polygalacturanase, ACC Oxidase, carbohydrate composition & storage, ADP glucose, pyrophosphatase. antibodies, edible vaccines

 

15.00
Unit IV: 
Choloroplast Transformation and Secondary Metabolites Production
  • Choloroplast Transformation: Advantages, vectors, Use of reporter genes, 35 S and other promoters, Plant secondary metabolites: types and mechanism of synthesis, alkaloids, terpenes, tannins, lignans, pigments, lipids, control mechanisms & manipulation of phenylpropanoid pathway. Purification strategies, Oleosin partitioning technology.

 

9.00
Unit V: 
Seed Biology
  • Seed biology, technology &role in agriculture, seed certification, seed banks, terminator gene technology & implications, biosafety & GM food crops

 

ESSENTIAL READINGS: 

ESSENTIAL READINGS: 

  • Brown T. A. (2016). Gene Cloning and DNA Analysis: An Introduction. Wiley Blackwell. 7th ed.
  • Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th edition. Blackwell Publishing, Oxford, U.K
  • Bhojwani, S.S and Razdan, M.K. (2010). Plant Tissue Culture: Theory and Practice Elsevier Science.
  • Gupta, P.K. (2010). Elements of Biotechnology (3rd edition), Rastogi and Co., Merrut, India.
  •  Chawla, H. S. (2009). Introduction to Plant Biotechnology. 3rd edition C RC Press.
  •  Das, H.K. (2010). Text Book of Biotechnology. 4th edition, Wiley India Pvt. Limited
  • Plant, Genes and Crop Biotechnology. (2ndEditon), Marten J. Chrispeels & David E. Sadava,  Jones and Bartlett Publishers. 2003.
  • Biotechnology – Secondary Metabolites, K.G. Ramawat, J.M Merllon: ISBN 9780367453237 Published December 2, 2019 by CRC Press
  • Laboratory Manual for Plant Biotechnology, H.S Chawla Oxford & IBH publishing Co. Pvt Ltd. 2004
  • Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler, OUP Oxford 2008
  • Plant Tissue Culture – Techniques & Experiments, (2nd edition) Roberta. H. Smith.  Elsevier. 2000
  • Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler,  Oxford University Press. 2005
  • Plant Tissue Culture. S.S. Bhojwani and M.K Razdan,  Elsevier. 2004
  • Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov,  Springer (India) Pvt Ltd. 2000
  • Introduction to Plant Tissue Culture : M K Razdan. Second Edition. Science Publishers 2003
  • Plant Biotechnology and Genetics: Principles, Techniques, and Applications. 2nd Edition. Edited by C Neal Srewart. Jr. Publishers – Wiley 2016.

SUGGESTED READINGS:

  • Gupta, P.K. (2011). Plant Biotechnology, Rastogi Publishers, Meerut, India.
  • Slater, A., Scott, N. W., & Fowler, M. R. (2008). Plant Biotechnology: an Introduction to Genetic Engineering. Oxford: Oxford University Press.
  • Buchanan, B. B., Gruissem, W., & Jones, R. L. (2015). Biochemistry & Molecular Biology of Plants. Chichester, West Sussex: John Wiley & Sons.
  • Clark DP and Pazdernik NJ. (2009). Biotechnology-Applying the Genetic Revolution. Elsevier Academic Press, USA.
  •  Umesha, S. (2013). Plant Biotechnology. The Energy And Resources.

 

REFERENCES: 

e RESOURCES:

JOURNALS:

 

 

Academic Year: 
2022-2023
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