PLANT BIOTECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY

Paper Code:

DBTE 501B

Credits:

Contact Hours:

Objective:

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course the learner will:

CO43B : Learn, remember and discuss learn the fundamental of Plant Tissue Culture

CO44B : Use various techniques in Plant Tissue Culture.

CO45B : Analyze and design Protoplast isolation methods and somatic hybridization

CO46B: Acquaint and plan tools for genetic engineering and use various types of vectors

CO47B: Apply various Methods of gene transfer in research.

Interactive Lectures, Discussion, Tutorials, Reading assignments, Self-learning assignments, Effective questions, Giving tasks

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

12.00

Unit I:

Fundamentals of Plant Biotechnology

Introduction to PTC laboratory. Sterilization techniques. Plant Tissue Culture media. Concept of totipotency, Embryogenesis and organogenesis and their practical applications. Somaclonal variations and applications.

12.00

Unit II:

Types of Cultures I

Callus culture. Embryo culture and its applications, embryo rescue. Micropropagation, axillary bud, shoot- tip and meristem culture to produce virus free plants. Anther culture, Pollen culture. Single cell and suspension cultures.

12.00

Unit III:

Types of Cultures II

Protoplast isolation: Principles and applications. Testing of viability of isolated protoplasts. Various steps in the regeneration of protoplasts. Somatic hybridization. Practical applications of somatic hybridization (Hybrids vs. Cybrids)

12.00

Unit IV:

Recombinant DNA Technology I

Gene cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC; Enzymes used in gene manipulation- Restriction endonucleases, DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase.

12.00

Unit V:

Recombinant DNA Technology II

Methods of gene transfer, Making and applications of genomic and c-DNA libraries; Blotting.Techniques- Southern, Northern and Western Blotting.

ESSENTIAL READINGS:

• Advanced Methods in Plant Biotechnology. David. R. Murray: Panima Publishing Corporation. 1996

• Plant, Genes and Crop Biotechnology. Marten J. Chrispeels & David E. Sadava: 2^nd Ed. Jones and Bartlett Publishers. 1999.

• Biotechnology in Crop improvement. H.S. Chawla: International Book Distributing Company, 1998.

• Practical Application of plant Molecular Biology. R.J. Henry: Chapman and hall.1997.

• Laboratory Manual for Plant Biotechnology. H.S Chawla: Oxford & IBH publishing Co. Pvt Ltd. 2003

• Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler: Oxford University Press. 2003

• Plant Tissue Culture – Techniques & Experiments Roberta. H. Smith: 2^nd ed. Elsevier. 2000.

• Brown, T. A., Gene Cloning and DNA Analysis, An Introduction. Wiley –Blackwell publication. 2010.

REFERENCES:

SUGGESTED READINGS:

• Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler: Oxford University Press. 2005

• Plant Tissue Culture. S.S. Bhojwani and M.K Razdan: Elsevier. 2004

• Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov: Springer (India) Pvt Ltd. 2000

• Plant Biotechnology. Hammond, P. McGarvey and V. Yusibov (Eds.) Springer Verlag: 2000.

• Plant Cell and Tissue Culture for the Production of Food ingredients. J. Fu, G. Singh, and W.R. Curtis (Eds): Kluwer Academic/Plenum Press.1999.

• Biotechnology – Secondary Metabolites. K.G. Ramawat, J.M Merllon: Oxford & IBH publishing house Co. Pvt. Ltd. 2000

• Plant Molecular Biology – A Practical Approach. C. H. Shaw: IRL Press Ltd. 1988.

• Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.

e RESOURCES:

• Tissue Culture Plants Techniques For Beginners | Agri Farming

• Agrobacterium-mediated gene transfer in plants and biosafety considerations - PubMed (nih.gov)

• Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool - PMC (nih.gov)

• Somatic Cell Hybridization | SpringerLink

• https://indiabioscience.org/media/articles/DBT-Life-Science-Protocol-Manual.pdf

• https://experiments.springernature.com/articles/10.1385/0-89603-127-6:499

• https://www.biologydiscussion.com/plants/plant-tissue-culture/experiments-on-plant-tissue-culture/57409

• https://www.cambridge.org/ca/9780521478922

• https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793(86)80556-7

• https://nptel.ac.in/courses/102103013

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