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PLANT BIOTECHNOLOGY AND RECOMBINANT DNA TECHNOLOGY
This course will enable the student to grasp plant molecular biology intricacies, harness recombinant DNA techniques and learn tissue culture techniques.
Introduction to PTC laboratory. Sterilization techniques. Plant Tissue Culture media. Concept of totipotency, Embryogenesis and organogenesis and their practical applications. Somaclonal variations and applications.
Callus culture. Embryo culture and its applications, embryo rescue. Micropropagation, axillary bud, shoot- tip and meristem culture to produce virus free plants. Anther culture, Pollen culture. Single cell and suspension cultures.
Protoplast isolation: Principles and applications. Testing of viability of isolated protoplasts. Various steps in the regeneration of protoplasts. Somatic hybridization. Practical applications of somatic hybridization (Hybrids vs. Cybrids)
Gene cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC; Enzymes used in gene manipulation- Restriction endonucleases, DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase.
Methods of gene transfer, Making and applications of genomic and c-DNA libraries; Blotting Techniques- Southern, Northern and Western Blotting.
- Advanced Methods in Plant Biotechnology. David. R. Murray: Panima Publishing Corporation. 1996
- Plant, Genes and Crop Biotechnology. Marten J. Chrispeels & David E. Sadava: 2nd Ed. Jones and Bartlett Publishers. 1999.
- Biotechnology in Crop improvement. H.S. Chawla: International Book Distributing Company, 1998.
- Practical Application of plant Molecular Biology. R.J. Henry: Chapman and hall.1997.
- Laboratory Manual for Plant Biotechnology. H.S Chawla: Oxford & IBH publishing Co. Pvt Ltd. 2003
- Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler: Oxford University Press. 2003
- Plant Tissue Culture – Techniques & Experiments Roberta. H. Smith: 2nd ed. Elsevier. 2000.
- Brown, T. A., Gene Cloning and DNA Analysis, An Introduction. Wiley –Blackwell publication. 2010.
- Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler: Oxford University Press. 2005
- Plant Tissue Culture. S.S. Bhojwani and M.K Razdan: Elsevier. 2004
- Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov: Springer (India) Pvt Ltd. 2000
- Plant Biotechnology. Hammond, P. McGarvey and V. Yusibov (Eds.) Springer Verlag: 2000.
- Plant Cell and Tissue Culture for the Production of Food ingredients. J. Fu, G. Singh, and W.R. Curtis (Eds): Kluwer Academic/Plenum Press.1999.
- Biotechnology – Secondary Metabolites. K.G. Ramawat, J.M Merllon: Oxford & IBH publishing house Co. Pvt. Ltd. 2000
- Plant Molecular Biology – A Practical Approach. C. H. Shaw: IRL Press Ltd. 1988.
- Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.
