PLANT TISSUE CULTURE

Paper Code: 
BTE 501
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to-

  • learn the fundamental of Plant Tissue Culture
  • get acquainted with various techniques in Plant Tissue Culture
  • analyze the genetic manipulations in Plant Tissue Culture
  • study the industrial application of Plant Tissue Culture
8.00
Unit I: 
Fundamentals

Introduction to in vitro Methods. Terms and definition. Use of growth regulators and fertilization.

Beginning of in vitro cultures in our country. Plant Tissue Culture media.

Embryo culture, embryo rescue after hybridization and its applications.

Introduction to the processes of embryogenesis and organogenesis and their practical applications.

7.00
Unit II: 
Various techniques- I

Clonal multiplication of elite species (Micropropagation) axillary bud, shoot- tip and meristem culture.

Haploids and their applications. Somaclonal variations and applications.In vitro pollination and fertilization.

Endosperm culture and production of triploids.

Single cell suspension cultures and their applications in selection of variants/mutants with or without mutagen treatment (of haploid culture preferably)

8.00
Unit III: 
Various techniques- II

Introduction to protoplast isolation: Principles and applications.

Testing of viability of isolated protoplasts

Various steps in the regeneration of protoplasts

Various methods for fusing protoplasts: Chemical and Electrical

Somatic hybridization

12.00
Unit IV: 
Applied aspects

Use of markers for selection of hybrid cells.

Practical applications of somatic hybridization (Hybrids vs. Cybrids)

Use of plant cell, protoplasts and tissue culture for genetic manipulation of plants.

10.00
Unit V: 
Genetic manipulation

Introduction of A. tumefaciens

Tumor formation on plants using A. tumefaciens(Monocots vs. Dicots)

Root – Formation using A. rhizogenes

Practical application of genetic transformation

ESSENTIAL READINGS: 
  1. Advanced Methods in Plant Biotechnology. David. R. Murray: Panima Publishing Corporation. 1996
  2. Plant, Genes and Crop Biotechnology.  Marten J. Chrispeels & David E. Sadava:  2nd Ed. Jones and Bartlett Publishers. 1999.
  3. Biotechnology in Crop improvement. H.S. Chawla:  International Book Distributing Company, 1998.
  4. Practical Application of plant Molecular Biology. R.J. Henry: Chapman and hall.1997.
  5. Laboratory Manual for Plant Biotechnology. H.S Chawla: Oxford & IBH publishing Co. Pvt Ltd. 2003
  6. Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler:  Oxford University Press. 2003
  7. Plant Tissue Culture – Techniques & Experiments Roberta. H. Smith: 2nd ed. Elsevier. 2000
REFERENCES: 
  1. Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler:  Oxford University Press. 2005
  2. Plant Tissue Culture. S.S. Bhojwani and M.K Razdan:  Elsevier. 2004
  3. Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov:  Springer (India) Pvt Ltd. 2000
  4. Plant Biotechnology.  Hammond, P. McGarvey and V. Yusibov (Eds.) Springer Verlag: 2000.
  5. Plant Cell and Tissue Culture for the Production of Food ingredients. J. Fu, G. Singh, and W.R. Curtis (Eds): Kluwer Academic/Plenum Press.1999.
  6. Biotechnology – Secondary Metabolites. K.G. Ramawat, J.M Merllon:  Oxford & IBH publishing house Co. Pvt. Ltd. 2000
  7. Plant Molecular Biology – A Practical Approach. C. H. Shaw:  IRL Press Ltd. 1988.
Academic Year: 
2014-2015
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