Paper IV: Advanced Plant Biotechnology (Advance Paper-II)

Paper Code: 
LSC-144 D
Credits: 
4
Contact Hours: 
60
12.00
Unit I: 
RECOMBINANT DNA TECHNOLOGY -1

Principles and methods of recombinant DNA technology-hybridization, Cloning strategies:Cutting and joining DNA molecules, c DNA and genomic DNA libraries , screening strategies, cloning in E. coli, plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors.

12.00
Unit II: 
RECOMBINANT DNA TECHNOLOGY – II

Polymerase chain reaction (PCR), DNA sequencing, Molecular markers- Non PCR based: RFLP, PCR based: RAPD, AFLP, SSR or Microsatellites, DNA Fingerprinting Genome projects-Arabidopsis genome, Rice Genome.

12.00
Unit III: 
TRANSGENESIS

Concept and history of transgenesis in plants, principles and techniques of gene cloning: direct DNA transfer to plant cells, Agrobacterium mediated transformation: Ti plasmid, process of T-DNA transfer and integration, vectors, promoter, terminator, marker and reporter genes, ethical and ecological issues of transgenesis, terminator technology (GURT)

12.00
Unit IV: 
TRANSGENICS IN CROP IMPROVEMENT

Resistance to Biotic Stress: Insect Resistance, Virus resistance and Disease resistance; Resistance to Abiotic stresses; Herbicide resistance; Transgenics for Quality improvement: improved storage, longer life flowers, flower colour and shape and male sterility; commercial transgenic crops.

12.00
Unit V: 
INTELLECTUAL PROPERTY RIGHTS

History and evolution of IPR, forms of IPR: patent, design and copyright; biotechnological Patent Facilitating Cell (BPFC), distinction    among various forms of IPR, rights/ protection, infringement or violation remedies against infringement (civil and criminal), Indian Patent Act.

ESSENTIAL READINGS: 

Books Recommended:

  • Altaian, A. Agricultural Biotechnology. CRC Press
  • Bhojwani, SS. 1990. Plant Tissue Culture: Applications and Limitations.
  • Bhojwani, SS and Razdan, MK. 1996. Plant Tissue Culture: Theory and Practice (a revised edition). Elsevier Science Publishers. New York, USA.
  • Brown, TA. 1999.Genomes. John Wiley & Sons (Asis) Pvt. Ltd., Singapore.
  • Callow, JA; Ford-Lloyd, BV and Newbury, HJ. 1997. Biotechnology and Plant
  • Genetic Resources: Conservation and Use. CAB International, Oxon, UK.
  • Chawla, HS. 2002. Introduction to Plant Biotechnology. Oxford & IBM Publishing Co. Pvt. Ltd.
  • Chrispeels, MJ and Sadava, DE. 1994. Plants, Genes and Agriculture. Jones and Barlett Publishers. Boston, USA.
  • Collins, HA and Edwards, S. 1998. Plant Cell Culture. Bios Scientific Publishers.

Oxford, UK.

  • Gustafson, JP. 2000.Genomes. Kluwer Academic Plenum Publishers. New York.
  • Jolles, O and Jomvall, H.(eds.) .2000. Proteomics in Functional Genomics. Birkhauser Verlag, Basel, Switzerland.
  • Kartha, KK. 1985. Cryopreservation of Plant Cells and Organs.
  • Primrose, SB. 1995. Principles of Genome Analysis. Blackwell ScienceLtd. Oxford, UK.
  • Edwards and Sue. 1998. Plant Cell Culture. Bios Scientific Publishers,    London.
  • Jane, B. Agricultural Biotechnology. Diane Publishing Co. Kumar, U. 2001. Methods in Plant Tissue Culture. Agrobios, Jodhpur.
  • Ignacimuthu, S. 2001. Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Murphy and Terence, M. 1998. Plant Biology. Wadsworth Publishing Co. California.
  • Narayanswamy. 1994. Plant Cell and Tissue Culture. Tata McGraw Hill, New Delhi
  • Shantharam, S and Montgomery, JF. 1999. Biotechnology, Biosafety and

Biodiversity. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi

  • Singh and Rita. 2004. Plant Biotechnology. Global Vision Publishing House
Academic Year: 
2018-2019
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