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LABORATORY II
Course Outcomes (COs):
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Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Upon completion of the course the learner will: CO 70: Learn experiments related to Molecular Biology and Genetics. CO 71: Learn demonstrations and experiments related to Immunology CO 72: Learn experiments related to Plant tissue Culture and applications |
Hands-on practical, Interactive Lectures, Discussion, Demonstrations, Simulation |
Class test, Semester end examinations, Hands-on practical assessment |
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1. Study of mitosis and meiosis.
2. X – Chromosome detection through Barr body experiment.
3. To study mitosis in onion root tips
4. To study meiosis in onion buds
5. Isolation of plasmid DNA
6. Isolation of genomic DNA
7. Spectrophotometric quantitation of nucleic acids
8. Experimental verification that absorption at OD260 is more for denatured DNA than native ds DNA
9. Demonstration of lac operon
10. Blood film preparation and identification of cells.
11. Ouchterlony double diffusion
12. Dot ELISA
13. Demonstrations:
o Sandwich ELISA
o Latex agglutination
o Rocket Immunoelectrophoresis
14. Restriction digestion of λDNA
15. Ligation
16. Transformation of DH5α (including preparation of competent cells)
17. Demonstartions:
18. Conjugation
19. Reporter gene assay (GFP)
20. Preparation of media.
21. Explant inoculation
22. Callus induction
23. Protoplast isolation and fusion
24. Synthetic seed preparation
25. Study of mutagens by Ames test
26. Blood Film preparation and identification of cells
27. Any other practical based on theory syllabus
