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Fermentation Technology and Recombinant DNA Technology (Theory)
The course will develop in depth understanding of fermenter design, operation, process parameters and recovery methods. In addition, it will also acquaint them to tools of genetic engineering and gene transfer techniques.
Bioreactors: Basic Design, Conventional and Specialized bioreactors (pulsed, fluidized, photo bioreactors), Types of fermentation processes batch, fed batch and continuous cultures. Primary and Secondary screening techniques.
Raw materials used in media production, inoculum preparation, industrial sterilization, Probes for process monitoring and control of fermentation parameters (temperature, pH, dissolved oxygen, foam).
Cell disruption, filtration, centrifugation, liquid-liquid extraction, solvent extraction (distillation), preparative ion exchange chromatography, drying.
Gene cloning vectors- Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC; Enzymes used in gene manipulation- Restriction endonucleases, DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase.
Methods of gene transfer, Making and applications of genomic and c-DNA libraries; Blotting Techniques- Southern, Northern and Western Blotting.
- Principles of Fermentation Technology by P. Stanbury, A. Whitaker, S. Hall. 3rd edition. Butterworth-Heinemann. 2016.
- Fermentation Microbiology and Biotechnology edited by E.M.T. El-Mansi, C.F. Bryce, A.L. Demain, A.R. Allman. 3rd edition. CRC Press. 2012.
- Bioprocess Engineering: Basic Concepts by M. L. Shuler, F. Kargi, 2 nd edition. Pearson Education India. 2015.
- Brown, T. A., Gene Cloning and DNA Analysis, An Introduction. Wiley –Blackwell publication. 2010.
SUGGESTED READINGS:
- Roger G. Harrison, Paul W. Todd, Scott R. Rudge, Demetri P. Petrides, Bioseparations Science and Engineering, 2nd Ed., Oxford University Press, 2003.
- Pauline M. Doran, Bioprocess Engineering Principles, 2nd Ed., Academic Press, 2012
- James Bailey, David Ollis, Biochemical Engineering Fundamentals, 2nd Ed., McGraw Hill Education, 2017.
- Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.
e RESOURCES:
- https://explore.bryanu.edu/
- Fermentation and Enzyme Technology (PTT 452/3) – LibGuides
- https://elibrary-data.imf.org.egateway.vit.ac.in/bibilo/principles-fermentation-technology
- https://www.coursera.org/lecture
- https://worldwidescience.org/topicpages/f/fermentation
- https://nptel.ac.in/courses/102103013
JOURNALS:
- Journal of Fermentation Technology
- Journal of Industrial Microbiology and Biotechnology
- International Journal of Molecular Biotechnology
- Journal of Fermentation and Bioengineering
