Fermentation Technology (Theory)

Paper Code: 
25MBL320A
Credits: 
02
Contact Hours: 
30
Objective: 

This course will enable the students to analyse the different types of fermentation processes and acquire the skills of isolation, preservation and improvement of industrially important organisms.

 

Course Outcomes: 

Course

 Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25MBL320A

 

Fermentation Technology

(Theory)

 

CO164A: Relate with general formulation with medium and other requisites for fermentation

CO165A:Appraise with general microbial technology

CO166A: Correlate various pharmaceutical applications of microbial technology                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

CO167A:Assess the industrial applications of various microorganisms in production of organic feed stocks, acids amino acids and vitamins

CO168A:Formulate production of antibiotics, enzymes and microbial cells

CO169A: Contribute effectively in course-specific interaction

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

4.00
Unit I: 
Fermentation media and scale up of fermentation

Medium formulation, energy source, carbon sources, nitrogen sources, minerals, growth factors, buffers, inhibitors and precursors, antifoaming agents. Air and medium sterilization. Inoculum preparation and scale up of fermentations.

 

 

7.00
Unit II: 
Microbial technology -I

Microbial technology in human welfare; Isolation and screening of microbes important for industry – advances in methodology and its application; Advanced genome and epigenome editing tools (e.g., engineered zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for genome editing, transcription factors for epigenome editing, and other emerging tools) for manipulation of useful microbes/strains and their applications.

7.00
Unit III: 
Microbial technology-II

Recombinant protein and pharmaceuticals production by microbes – common bottlenecks and issues (technical/operational, commercial and ethical); Attributes required in industrial microbes (Streptomyces sp., Yeast) to be used as efficient cloning and expression hosts (biologicals production); Microbial cell factories.

 

 

6.00
Unit IV: 
Industrial application of microorganism-I

Concept of primary and secondary metabolites;Industrial production of organic feed stocks- ethanol; acetone/ butanol fermentations; organic acids- citric acid, acetic acid, amino acids- glutamic acid, lysine, vitamins- riboflavin; Microbial transformation of steroids.

 

6.00
Unit V: 
Industrial application of microorganism-II

Industrial uses of molds; Industrial production of antibiotics- penicillin, streptomycin, tetracycline; Enzymes as fermentation products- amylases, proteases; Techniques of enzyme immobilization; Microbial cells as fermentation products- commercial production of bakers’ yeast, mushrooms and algae.

 

 

ESSENTIAL READINGS: 
  • Principles of Fermentation Technology by P. Stanbury, A. Whitaker, S. Hall. 3rd edition. Butterworth-Heinemann. 2016.
  • Fermentation Microbiology and Biotechnology edited by E.M.T. El-Mansi, C.F. Bryce, A.L. Demain, A.R. Allman. 3rd edition. CRC Press. 2012.
  • Bioprocess Engineering: Basic Concepts by M. L. Shuler, F. Kargi, 2nd edition. Pearson Education India. 2015.
  • Lee, Y. K. (2013). Microbial Biotechnology: Principles and Applications. Hackensack, NJ: World Scientific.
  • Moo-Young, M. (2011). Comprehensive Biotechnology. Amsterdam: Elsevier.
  • Nelson, K. E. (2015). Encyclopedia of Metagenomics. Genes, Genomes and Metagenomes: Basics, Methods, Databases and Tools. Boston, MA: Springer US.

      SUGGESTED READINGS:

  • Modern Industrial Microbiology & Biotechnology by N. Okafor. 1st edition. CRC Press, USA 2007.
  • Microbial Biotechnology: Fundamentals of Applied Microbiology by A.N. Glazer, H. Nikaido. 2nd edition. Cambridge University Press 2007.
  • Process Biotechnology Fundamentals (2nd edition by S.N. Mukhopadhyaya. Vivi Books Pvt. Ltd 2004
  • Manual of Industrial Microbiology and Biotechnology (2nd edition). Edited by A.L. Demain and J. E Davies, Panima Publications 2004

 

REFERENCES: 

e RESOURCES:

      JOURNALS:

  • Journal of Fermentation Technology
  • International Journal of Food and Fermentation Technology

 

 

Academic Year: 
2025-2026
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