Molecular Genetics & Genomics

Paper Code: 
BIF 324
12.00
Unit I: 
Genome Basics

Chromosomes structure and Genome Organization. Structure of Prokaryotic & Eukaryotic Genes, Transposable Genetics Elements, Repetitive elements, LINE and SINES, VNTRs, Central Dogma of Molecular Biology

10.00
Unit II: 
DNA technology

Cloning strategy, Genetic and physical mapping, molecular markers, PCR Primer & Oligos Designiing,

12.00
Unit III: 
Genomics

Human Genome Project, Current techniques: Sanger’s method & Maxam- Gilbert method. Next Generation Sequencing Techniques, Sequence Assembly and Annotation, ORFs, Gene Identification and Prediction Methods, Genome Alignment and Comparative Genomics. Pyrosequencing & its modifications: Solexa, 454 sequencing.

14.00
Unit IV: 
Microarray

Microarray Basics, DNA Hybridization, DNA & Protein chips. Experimental Design: Features, target Preparation, Labeling & Sensitivity, Spotting media. Microarray Data analysis: image and raw data, Preprocessing & Normalization methods, Pattern Finding & Clustering. Statistical Tests and differential gene expression analysis, Applications of Microarray Technology.

12.00
Unit V: 
Microarray Databases and Tools

GEO, YMD, SMD, ArrayExpress etc. SNOMAD, magic tool(open software). Bioconductor, Commercial & Open Source tools for microarray.

ESSENTIAL READINGS: 

1) Genes VII, Latest Edition. 2) TA Brown. “Genomes”. 2nd Edition, Bios Scientific Publishers Ltd., 2002. 3) “Microarray Gene Expression Data Analysis: A Beginner's Guide” (ISBN: 978-1-4051-0682-5). 4). Dov Stekel. “Microarray Bioinformatics.” (ISBN:9780521670500).

Academic Year: 
2018-2019
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