Analytical Techniques

Paper Code: 
BTE 102
Credits: 
03
Contact Hours: 
45
Objective: 

This paper will enable students to- 

      

  1. Learn various techniques used in Biotechnology for experimentation. 
  2. The students will be able to understand the basic principles and working of different instruments present in a Biotechnology lab and their applications. 

 

Course Outcomes (COs):

 

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment

 Strategies 

Paper Code

Paper Title

BTE 102

Analytical

Techniques

CO 6    Explain the various separation techniques and its instrumentation.

 

CO 7    Understand various fundamentals of spectroscopy, qualitative and quantitative analysis and characterize functionalities of biomolecules by using spectroscopic techniques. 

 

 

CO 8    Understand Principle, working, ray diagram and application of advance microscopes 

 

CO 9 Understand different separation techniques

Approach in teaching: Interactive

Lectures,

Discussion, Tutorials, Reading assignments, presentations

 

Learning activities for the students: Self-learning assignments, Effective questions, Seminar presentation, Giving tasks.

C A test,

Semester end examinations, Quiz, Solving problems in

tutorials, Assignments, Classroom interaction

 

9.00
Unit I: 
Microscopy
  • Introduction to basic principle and applications: Light microscopy (bright field, dark field, phase contrast and fluorescence microscopy), electron microscopy (TEM and SEM)

 

10.00
Unit II: 
Optical methods of Analysis
  • Introduction to basic principles and applications: Beer Lambert’s Law               
  • Spectrophotometry (UV-visible, infra red, AAS), Mass spectroscopy 

 

10.00
Unit III: 
Chromatography
  • Working principle, instrumentation and application of:  Paper Chromatography, Thin Layer Chromatography, Column Chromatography (Affinity Chromatography, Ion-exchange Chromatography, Gel filtration Chromatography or Exclusion Chromatography)

 

10.00
Unit IV: 
Electrophoretic Techniques
  • Principle, factors affecting electrophoresis- pH, voltage, supporting medium (agar, polyacrylamide, dextran), Agarose gel electrophoresis, PAGE, SDS-PAGE, isoelectric focusing

 

8.00
Unit V: 
Centrifugation

 

  • Centrifugation: principle, differential and density gradient centrifugation, ultracentrifugation (preparative and analytical centrifuges) sedimentation analysis & RCF 

 

 

ESSENTIAL READINGS: 

 

  • Methods in Modern Biophysics, B Notting, Springer Verlag Berlin Heidelberg New York, 2003
  • Principles and Techniques  of Biochemistry and  Molecular Biology, 6th edition,  K Wilson and J Walker (ed.), Cambridge University Press, 2007
  • Bioinstrumentation, J G Webster,  John Wiley & Sons Inc. 2004     
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
  • Spectroscopy for the Biological Sciences, G G Hames, John Wiley & Sons Inc. 2005   

 

Academic Year: 
2021-2022
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