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Genetic Engineering
- learn the basics of Genetic Engineering
- study different cloning and expression vectors
- study the enzymes used in Genetic Engineering
- learn the procedures involved in isolation of genomic and nuclear DNA
Milestones & Importance of Genetic Engineering, vehicles for gene cloning: Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC.
Introduction of foreign DNA into plant and animal cells.
Enzymes used in G.E: DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase, Reverse transcriptase, Restriction endonucleases, Nick translation system.
Techniques: PCR, Colony and Plaque hybridization, Northern, southern, Western hybridization, Dot and slot Blot technique, DNA sequencing: Maxam Gilbert method, Sanger’s method, automated DNA sequencing
Production of protein from cloned genes, recombinant insulin, growth hormones, human proteins, recombinant vaccines, genetic counseling.
In Agriculture: Insect resistance, Virus resistance, modification of seed protein quality, suppression of endogenous genes, male sterility, biochemical production, plant derived vaccines, In Forensic science: identification of crime suspects, kinship analysis
- S.B.Primorse, (1994) Molecular Biotechnology (2nd Edn.), Blackwell Scientific Publishers, Oxford.
- M.R.Walker and R.Repley, (1997). Route Maps in Gene Technology, Blackwell Science Ltd.Oxford.
- T.A. Brown ,(2001). Gene Cloning and DNA Analysis. An Introduction. Blackwell Science.
- Sambrook J., Fritsch E.F and Maniatis T. (2000) Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory Press, New York.
- Glover D.M. and Hames B.D. (1955) DNA Cloning: a practical Approach, , IRL Press Oxford.
- Kaufman P.B., Kim W.Wu. D.and Cseke L.J, (1995). Molecular and Cellular Methods in Biology and Medicine, CRC Press. Florida,.
- Berger S.L. and Kimmel A.R. (1998). Methods in Enzymology, Guide to Molecular Cloning Techniques, Academic press. San Diego,
- Gooddol D.V. (1990). Methods in Enzymology, Gene Expression Technology, Academic Press, San Diego.
- Mickloss D.A.and Greyer G.A. (1990). DNA Science. A First Course in Recombinant Technology, ,Cold Spring Harbor Laboratory Press, New York,
- Davies J.A and Roznikolf W.S. (1992). Milestones in Biotechnology. Classic papers on genetic Engineering, Butterworth-Helnemann, Boston,.
- Kingsman S.M. and Kingsman A.J (1998). Genetic Engineering. An Introduction to gene analysis and exploitation in eukaryotes. Blackwell Scientific Publications, Oxford, 1998.
