GENETIC ENGINEERING

Paper Code: 
BTE 402
Credits: 
3
Contact Hours: 
45
Objective: 

Upon completion of the course learners will be able to:

CO 51: Learn the basics of Genetic Engineering and study different cloning and expression vectors which will help to build their concrete concepts about the subject.

CO 52: Apply enzymes used in Genetic Engineering in the procedures involved in isolation of genomic and nuclear DNA.

CO 53: Use tools and techniques of the PCR, hybridization, DNA sequencing procedures for creating new and improving existing issues in the field of medicine.

CO 54: Apply tools and techniques for the production of therapeutic proteins, solve cases by genetic counselling and vaccine development.

CO 55: Apply their knowledge for improving and developing various crop varieties in the field of agriculture.

CO 56: Solve issues related to Forensic science, improvement of existing industrial processes and contribute to society as a whole.
 

 

8.00
Unit I: 
Introduction to Genetic Engineering
  • Milestones & Importance of Genetic Engineering, vehicles for gene Cloning: Plasmids, Bacteriophages, Cosmids, phagemids, YAC, BAC.

 

 

10.00
Unit II: 
Tools & Techniques- I
  • Introduction of foreign DNA into plant and animal cells.
  • Enzymes used in G.E: DNA polymerase, Polynucleotide kinase, ligase, Terminal deoxynucleotidyl transferase, Reverse transcriptase, Restriction endonucleases, Nick translation system.

 

 

9.00
Unit III: 
Tools & Techniques- II
  • Techniques: PCR, Colony and Plaque hybridization, Northern, southern, Western hybridization, Dot and slot Blot technique, DNA sequencing: Maxam Gilbert method, Sanger’s method, automated DNA sequencing.

 

 

9.00
Unit IV: 
Applications of Genetic Engineering – I
  • Production of protein from Cloned genes, recombinant insulin, growth hormones, human proteins, recombinant vaccines, genetic counseling.

 

 

9.00
Unit V: 
Applications of Genetic Engineering –II
  • In Agriculture: Insect resistance, Virus resistance, modification of seed protein quality, suppression of endogenous genes, male sterility, biochemical production, plant derived vaccines, In Forensic science: identification of crime suspects, kinship analysis

 

 

ESSENTIAL READINGS: 
  • Brown, T. A., Gene Cloning and DNA Analysis, An Introduction.  Wiley –Blackwell publication. 2010.
  • Sandy B. Primrose, Richard M. Twyman.  Principles of Gene Manipulation and Genomics, Blackwell Sciectific Publication. 2009.
  • Brown, T. A., Genomes(3rd ed.). New York: Garland Science Pub. 2006.
  • Old, R. W., Primrose, S. B., & Twyman, R. M., Principles of Gene Manipulation: an Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications. 2001.
  • Green, M. R., & Sambrook, J., Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 2012.

 

REFERENCES: 

 

SUGGESTED READINGS:

  • P.B. Kaufman, W. Wu. D. Kim and L.J; Cseke, Molecular and Cellular Methods in Biology and Medicine, CRC Press, Florida.
  • M. Glover and B.D. Hames, DNA Cloning: a Practical Approach, IRL Press, Oxford.
  • S.L. Berger and A.R. Kimmel, Methods in Enzymology vol. 152, Guide to Molecular Cloning Techniques, Academic Press, Inc. San Diego.
  • D.V. Goeddel, Methods in Enzymology Vol 185, Gene Expression Technology, Academic Press, Inc., San Diego.
  • Kingsman S.M. and Kingsman A.J., Genetic Engineering. An Introduction to gene analysis and exploitation in eukaryotes. Blackwell Scientific Publications, Oxford, 1990.
  • Desmond S.T. Nicholl, An Introduction to Genetic Engineering. Edited by, Cambridge University Press. 2002.
  • Sue Carson and Dominique Robertson, Manipulation and Expression of Recombinant DNA., Second edition, Academic Press. 2005.
  • Primrose and Twyman, Principles of Gene Manipulation and Genomics. (7th edition). Blackwell Publishers. 2006.

 

 

 

e RESOURCES:

 

JOURNALS:

  • International Journal of Molecular Biotechnology
  • Indian Journal of Biotechnology
  • Current Science

 

 

 

 

Academic Year: 
2022-2023
  • Printer-friendly version
  • PDF version