GENETIC ENGINEERING

• Scope and milestones in genetic engineering, Basic tools and techniques used in recombinant DNA technology: Restriction endonuclease, DNA modifying enzymes, cloning vectors: plasmids, bacteriophage, cosmid, phagemids, YAC, BAC, in vitro construction of vectors, expression vectors.
• Linkers and Adaptors. Principle and uses of nucleic acid hybridization. Principle and applications of polymerase chain reaction, Q-PCR.

• DNA and genomic library: m- RNA enrichment, construction and screening of c DNA and genomic library, Genome sequencing: strategies of genome sequencing, Sequencing vector, fluorescent tagging, Pyrosequencing, Automated DNA sequencing, Introduction to Next generation sequencing technologies.

• Genetic and physical mapping, Restriction mapping and map construction, Analysis of genetic variations: RAPD, RFLP, AFLP and other molecular marker techniques, application of RFLP in forensic studies, disease prognosis, genetic counselling, pedigreeanalysis.                                                                                                                     

Direct gene delivery methods and Agrobacterium mediated transformation techniques, Gene therapy: Target gene replacement, gene knockout technique, Genome editing Techniques (ZFN, TALEN, CRISPR/ Cas9), computer aided drug designing. Patenting of cloned life forms

• Site directed mutagenesis and protein engineering. in vitro DNA synthesis, in vitro transcription and translation. Heterologous gene expression in bacteria, yeasts, insects, mammals and plants, codon optimization, DNA and protein microarray technology, RNase protection assay, Reporter gene assay, Western blotting, S1 nuclease assay.