LABORATORY –I

Paper Code: 
MBL 103
Credits: 
02
Contact Hours: 
60
Objective: 

This paper will enable students to understand –

 

  1. Learn the principle and working of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules.

Course Outcomes (COs):

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

MBL 103

Laboratory I

CO 1 Understand the layout of a microbiology laboratory.

CO 2 To get acquainted with the various instruments in a microbiology/ biochemistry laboratory

CO 3 Understand various accessories for microbiology practical

CO 4 To make qualitative and quantitative description of the basic enzymatic phenomena and processes.

CO 5 Cultivate bacteria with different cultivation technique

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

CONTENTS

  1. Preparation of  buffers
  2. To determine the pH of solutions.
  3. To prepare molar and normal solutions
  4. Verification of Beer’s law
  5. Quantitation of proteins by Biuret assay
  6. Quantitation of carbohydrates by DNS method
  7. Quantitation of cholesterol
  8. Separation of given amino acid mixture by paper chromatography
  9. Separation of sugars by thin layer chromatography
  10. To study the working of light microscope and centrifuge
Academic Year: 
2020-2021
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