LABORATORY I

Paper Code: 
BTE 126
Credits: 
08
Contact Hours: 
240
Objective: 

This course will enable the students to -

  1. Make the students learn the practical aspect of Enzymology, Biochemistry and microbial growth and diversity
  2. Gain practical knowledge on Instrumentation, biostatistics and bioinformatics

Course Outcomes (COs):

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 126

 

Laboratory I

 

Upon completion of the course students will be able to:

CO 20. Learn experiments related  to  Enzyme Activity

CO 21.Learn  Hands on experience on Chromatography, Electrophoresis and Bioinformatics tools

CO 22.Perform experiments related to biochemistry

CO 23. Learn experiments related to microbial growth and diversity

Approach in teaching:

Discussion, Tutorials, Demonstration

Learning activities for the students:

Effective questions, Simulation, Giving tasks,  Practical

 

Class test, Semester end examinations

CONTENTS

  1. Microtomy of liver/intestine
  2. Histochemical localistion of starch, lipids and protein in liver.
  3. Verification of Beer Lambert’s Law
  4. Estimation of protein by Folin Lowry method
  5. Estimation of protein by Biuret test
  6. Estimation of reducing sugar by DNSA method
  7. Estimation of sugar by Anthrone reagent
  8. Estimation of glycogen from the given tissue
  9. Estimation of cholesterol from the given tissue
  10. To study the effect of substrate concentration on enzyme activity
  11. To study the effect of temperature on enzyme activity
  12. To study the effect of pH on enzyme activity
  13. To assay the activity of acid phosphatase
  14. Separation and identification of amino acids by TLC
  15. Separation of protein by SDS-PAGE
  16. Demonstrations:
    1.  Gel Filtration Chromatography
    2. Ion Exchange Chromatography
    3. Descending Paper Chromatography
    4. Column Chromatography
    5. Affinity Chromatography
    6. Western Blotting
  17. Preparation of liquid and solid media for growth of microorganisms.
  18. Isolation and maintenance of organisms by plating, streaking and serial dilution methods, slants and stab cultures, storage of microorganisms.
  19. Isolation of pure cultures from soil and water.
  20. Growth Kinetics, Effect of temperature, pH and carbon and nitrogen source on growth.
  21. Enumeration by standard plate count.
  22. Microscopic examination of bacteria, yeast and molds and study of organisms by Gram stain, Negative staining, staining for spores etc.
  23. Isolation of Rhizobia
  24. Isolation of Actinomycetes
  25. Biochemical characterization of selected microbes.
  26. Biostatistics exercises
  27. Operating systems (DOS)
  28. Information searching on Internet.
  29. Sequence retrievals form NCBI, Genbank, EMBL, DDBJ

Any other practical based on theory syllabus.

Academic Year: 
2019-2020
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