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Laboratory I
This course will enable the students to –
- Learn the principle and wrking of different instruments.
- Learn good laboratory practices.
- Qualitative and quantitative estimation of various biomolecules.
Course Outcomes (COs):
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Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BTE 103 |
Laboratory I |
CO 10 Understand the layout of a microbiology laboratory.
CO 11 To get acquainted with the various instruments in a microbiology/ biochemistry laboratory
CO 12 Understand various accessories for microbiology practical
CO 13 to make qualitative and quantitative description of the basic enzymatic phenomena and processes.
CO 14 Cultivate bacteria with different cultivation technique |
Approach in teaching: Interactive Lectures, Discussion, Practical experiments
Learning activities for the students: Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.
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C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction |
CONTENTS
- Preparation of buffers
- To determine the pH of solutions.
- To prepare molar and normal solutions
- Verification of Beer’s law
- Quantitation of proteins by Biuret assay
- Quantitation of carbohydrates by DNS method
- Quantitation of cholesterol
- Separation of given amino acid mixture by paper chromatography
- Separation of sugars by thin layer chromatography
- To study the working of light microscope and centrifuge
