Laboratory I

Paper Code: 
BTE 103
Credits: 
02
Contact Hours: 
60
Objective: 

This course will enable the students to –

 

  1. Learn the principle and wrking of different instruments.
  2. Learn good laboratory practices.
  3. Qualitative and quantitative estimation of various biomolecules. 

 

Course Outcomes (COs):

 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment

Strategies 

Paper Code

Paper Title

BTE 103

Laboratory I

CO 10 Understand the layout of a microbiology laboratory.

 

CO 11 To get acquainted with the various instruments in a microbiology/ biochemistry

laboratory 

 

 

CO 12 Understand various accessories for microbiology practical

 

CO 13 to make qualitative and quantitative description of the basic enzymatic phenomena and processes.

 

 

CO 14 Cultivate bacteria with different cultivation technique

Approach in teaching: Interactive

Lectures,

Discussion, Practical experiments

 

Learning

activities for the students: Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test,

Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

CONTENTS

 

  1. Preparation of  buffers
  2. To determine the pH of solutions.
  3. To prepare molar and normal solutions
  4. Verification of Beer’s law
  5. Quantitation of proteins by Biuret assay
  6. Quantitation of carbohydrates by DNS method
  7. Quantitation of cholesterol
  8. Separation of given amino acid mixture by paper chromatography
  9. Separation of sugars by thin layer chromatography
  10. To study the working of light microscope and centrifuge

 

 

Academic Year: 
2021-2022
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