Molecular Biology & Microbial Genetics

Paper Code: 
MBL 301
Credits: 
3
Contact Hours: 
45
Objective: 
  • Understand the basic mechanism involved in the flow of genetic information
  •  Understand molecular mechanism of mutations and DNA repair
  •  Understand recombination in bacteria
  •  Have an idea of cloning vectors
10.00
Unit I: 
DNA Structure and Replication

                                                                           

DNA as a genetic material, structure of DNA and RNA, Types of DNA (A, B, Z), DNA replication in prokaryotes and eukaryotes.

 

8.00
Unit II: 
Transcription

                                                                                             

Mechanism of Transcription in prokaryotes and eukaryotes, enzymes and transcription factors, Posttranscriptional modifications of eukaryotic mRNA.

 

10.00
Unit III: 
Translation and Regulation of Gene Expression

 

The genetic code, Translational machinery in eukaryotes and prokaryotes, inhibitors of protein synthesis

Regulation of Gene expression in prokaryotes (lac and trp operon)

10.00
Unit IV: 
Mutations and Mutant Selection

                                      

Molecular mechanism of mutation, forward and reverse mutation, transition, transversion, Spontaneous and induced mutations

Methods for mutant selection- direct selection, antibiotic selection, replica plating

Mechanism of DNA repair-photo reactivation, Nucleotide excision repair, mismatch repa

 

7.00
Unit V: 
Recombination in Bacteria

                                                                     

Transformation, transduction and conjugation

A brief idea of cloning vectors (plasmids, cosmids, bacteriophages).

ESSENTIAL READINGS: 
  1. Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2005.
  2. Gene VI, Lewin, B, New York, Oxford University Press, 2000.
  3. Cell and Molecular Biology, 4th edition, Gerald Karp, John Wiley and Sons. 2005.

 

 

REFERENCES: 

 

  1. Biochemistry and Molecular Biology, 3rd edition, Elliott W H and Elliot D C, Oxford University Press, 2005.
  2. Essentials of Molecular Biology, 3rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 1998.
  3. Instant Notes, Molecular Biology. Turner P.G., Mc Lennan, Bates A D, and White M R H, 2nd edition. Bios Scientific Publishers. 2002.
  4. Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons. 1987.
  5. Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 1994.
Academic Year: 
2015-2016
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