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Paper IV: Microbiology (Advance Paper-II)
Paper Code:
LSC 144 A
Credits:
04
Contact Hours:
60
12.00
Unit I:
TAXONOMY
Microbial evolution, systematic and taxonomy-Evolution of earth and earliest life forms; primitive organisms, their metabolic strategies and molecular coding; approaches to bacterial taxonomy, DNA-DNA and DNA-RNA hybridization, G+C content determination, ribotyping, Ribosomal RNA sequencing; Characteristics of primary domains, Taxonomy, Nomenclature and Bergey's Manual. Culture collection, maintenance and preservation of microorganisms.
12.00
Unit II:
EXTREMOPHILES
halophiles halophilic thermophiles.
Heat, cold and deep sea adaptations. Exploitation of extremophiles in medicine, agriculture and industries
12.00
Unit III:
DETECTION, ENUMERATION AND IDENTIFICATION OF MICROBES
Environmental sample collection and processing. Microscopic techniques: Fluororescence (direct count, fluorescent immunolabelling, FISH . Electro Microscopy: Scanning Transmission, elemental analysis), Confocal Laser scanning microscopy, Scanning Probe microscopy ( atomic force microscopy), flow cytometry
Culture methods: isolation and enumeration of bacteria, media for bacteria, culture methods of algae, fungi and viruses.
Physiological methods: carbon respiration, incorporation of radiolabelled traces into cellular macromolecules, adenylate energy charges, enzyme assays stable isotope probing, functional genomics and proteomics –based approaches
12.00
Unit IV:
AQUATIC MICROBIOLOGY
Microbial habitats in aquatic environments (planktonic, Benthic, microbial mats, biofilms).Aquatic environments; (fresh water, brackish water, marine water, subterranean).
12.00
Unit V:
SOIL MICROBIOLOGY
Micro flora of various soil types, Culture methods for enumeration of soil microorganisms, physiochemical characteristics, soil as a microbial environment microorganism in surface soil, shallow & in the deep subsurface.
ESSENTIAL READINGS:
- Molecular Genetics of Bacteria, Larry Shyder and Wendy Champness, ASM Press,1997.
- Introduction to Geomicrobiology, Kurt Konhauser, Blackwell Publishing,2007.
- Environmental Biotechnology: Basic Concepts and Applications, Indu Shekhar Thakur, IK International, 2006.
- Medical Microbiology and Immunology: Examination and Board Review
- Warren Levinson and Ernst Jawetz, (7th edition), Mc Graw Hill, 2002.
- Microbial Diversity: Current Prospective and Potential Applications, T. Satyanarayana, B.N. Johri, IK International, 2005.
Academic Year:
