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Plant Tissue Culture
This course will enable the students to -
- Learn the fundamental of Plant Tissue Culture
- Get acquainted with various techniques in Plant Tissue Culture
- Analyze the genetic manipulations in Plant Tissue Culture
- Study the industrial application of Plant Tissue
Course Outcomes (COs):
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Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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CO 59 Learn the fundamental of Plant Tissue Culture
CO 60 Get acquainted with various techniques in Plant Tissue Culture |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, presentations Learning activities for the students: Self-learning assignments, Effective questions, Seminar presentation, Giving tasks. |
C A test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Classroom interaction |
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BTE 501 |
Plant Tissue Culture |
CO 61 Analyze the genetic manipulations in Plant Tissue Culture |
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CO 62 Study the industrial application of Plant Tissue Culture |
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CO 63 Learn the in-vitro method of production of secondary metabolites |
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- Brief history of Plant Tissue Culture (Contribution of Indian Scientist), Introduction to in vitro Methods. Terms and definition.
- Plant Tissue Culture media.Concept of totipotency Embryo culture, embryo rescue after hybridization and its applications.
- Introduction to the processes of embryogenesis and organogenesis and their practical applications.
- Micropropagation: axillary bud, shoot- tip and meristem culture,anther culture.
- Haploids and their applications. SomaCOnal variations and applications. In vitro pollination and fertilization. Endosperm culture and production of triploids.Single cell suspension cultures.
· Introduction to protoplast isolation: Principles and applications.
· Testing of viability of isolated protoplasts
· Various steps in the regeneration of protoplasts
· Various methods for fusing protoplasts: Chemical and Electrical
Somatic hybridization
- Use of markers for selection of hybrid cells.
- Practical applications of somatic hybridization (Hybrids vs. Cybrids)
- Use of plant cell and tissue culture for genetic manipulation of plants. Marker assisted plant breeding.
- Methods of gene transfer: Direct and Agarobacterium mediated. Introduction of A. tumefaciens and A. rhizogenes (Monocots vs. Dicots). Formation of hairy root culture. Practical application of genetic transformation .
- Advanced Methods in Plant Biotechnology. David. R. Murray: Panima Publishing Corporation. 1996
- Plant, Genes and Crop Biotechnology. Marten J. Chrispeels & David E. Sadava: 2nd Ed. Jones and Bartlett Publishers. 1999.
- Biotechnology in Crop improvement. H.S. Chawla: International Book Distributing Company, 1998.
- Practical Application of plant Molecular Biology. R.J. Henry: Chapman and hall.1997.
- Laboratory Manual for Plant Biotechnology. H.S Chawla: Oxford & IBH publishing Co. Pvt Ltd. 2003
- Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler: Oxford University Press. 2003
- Plant Tissue Culture – Techniques & Experiments Roberta. H. Smith: 2nd ed. Elsevier. 2000
- Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler: Oxford University Press. 2005
- Plant Tissue Culture. S.S. Bhojwani and M.K Razdan: Elsevier. 2004
- Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov: Springer (India) Pvt Ltd. 2000
- Plant Biotechnology. Hammond, P. McGarvey and V. Yusibov (Eds.) Springer Verlag: 2000.
- Plant Cell and Tissue Culture for the Production of Food ingredients. J. Fu, G. Singh, and W.R. Curtis (Eds): Kluwer Academic/Plenum Press.1999.
- Biotechnology – Secondary Metabolites. K.G. Ramawat, J.M Merllon: Oxford & IBH publishing house Co. Pvt. Ltd. 2000
- Plant Molecular Biology – A Practical Approach. C. H. Shaw: IRL Press Ltd. 1988.
