Virology, Mycology and Phycology

Paper Code: 
MBL 225
Credits: 
5
Contact Hours: 
75
Objective: 
  • To learn about classification and structure of viruses.
  • To know about general methods of diagnosis and serology.
  • Know about bacteriophages.
  • To understand mycology and phycology.
10.00
Unit I: 
General Virology

                                                                              

Brief outline on discovery of viruses, nomenclature and classification of viruses : distinctive properties of viruses; morphology & ultra structure; capsids & their arrangements; types of envelops and their composition-viral genome, their types and structures; virus related agents (viroids, prions)

15.00
Unit II: 
Cultivation, Diagnosis and serology

 

                                                 

 Cultivation of viruses in embryonated eggs, experimental animals , cell cultures; Primary & secondary cell cultures; suspension cell cultures and monolayer cell cultures; cell lines and transgenic systems; serological methods - heamagglutination & HAU; compliment fixation ;immunofluorescenemethods, ELISA and Radioimmunoassay; assay of viruses physical and chemical methods (Protein, nucleic acid, radioactivity, trackers, electron microscopy)-Infectivity assay (plaque method, end point method)- Infectivity of plant viruses.

20.00
Unit III: 
Animals and Plant Viruses

 

                                                            

An overview of replicative cycle of animal viruses

Plant viruses: Effect of viruses on plants, Appearance of plants; histology, physiology and cytology of plants; common virus disease of plants eg. paddy (Rice Tungro, Rice Hozablanca), tomato(Tomato Yellow Leaf Curl, Tomato Mottle) and sugarcane (Sugarcane Mosaic); Transmission of plant virus with vectors (insects, nematodes, fungi) and without vectors (contact, seed and pollens), Prevention of crop loss due to virus infection- virus free planting material; vector control.

15.00
Unit IV: 
Mycology

                                                    

                                                                                             

An Introduction to fungi-History, general features of fungi, Classification of fungi, according to Aiasworth and Sussman (1974) as per UGC classification with the general aspects of Major division of fungi Nutrition of fungi, Vitamin requirements, Homothallism, Heterothallism, Heterokaryosis, The Parasexual cycle, Sex Hormones in Fungi. Fungi as insect symbiont. Mycotoxins and Mycotoxicoses. Attack on fungi by other microbes, yeasts, moulds and viruses.

15.00
Unit V: 
Phycology

 

                                                                                                   

An introduction to Algae, General features and classification of algae. Occurrence, thallus organization and reproduction in chlorophyceae euglenophyceae, phaeophyceae, pyrrophyceae and diatoms.

Algal ecology & biotechnology. Economic importance of algae. Lichen, ascolichen, basidiolichen, deuterolichen

ESSENTIAL READINGS: 

1.     Introduction to Modern Virology, IV.  Dimmock NJ, Primrose SB Edition, Blackwell Scientific Publications, Oxford. 1994

2.     Virology-3rd Edition, Conrat HF, Kimball PC and Levy JA. Prentice Hall, Englewood cliff, New Jersey1994.

3.     Functionals of plant virology-  Mathews,RE.Academic press,San Diego.1992

4.     Text Book on principles of Bacteriology, virology and Immunology- Topley and Wilson. Edward Arnold, London. 1995

5.     Diagnostic procedures for viral and Rickettsial diseases.american public Health association, NY. Lennetter1984

6.     The genetics of Bacteria and their viruses- William Hayes. Blackwell Scientific Publishers, London. 1985

7.     Principles of microbiology- Ronald M.Atlas. Mosby Year Book, Inc. Missouri 63146. 1985

8.     Textbook of Microbiology- R. Ananthnarayan and CCJ Paniker. (6th Edition). Orient Longman Pvt Ltd. 2003

9.     An Introduction to Mycology, Mehrotra RS and Aneja KR, New Age International Publishers, 1990

10.  Fundamentals of Fungi, Moore E., Landeeker , Prentice Hall

REFERENCES: 

1.     Plant viruses- Kenneth M.Smith.Universal Book Stall,New Delhi.1999

2.     Bacteriophages- Mark H.Adams. Inter science publishers, Inc. New York.

3.     Applied Virology- D.G.A Walkey. International Books & Periodicals supply service.New Delhi. 1985

4.     Plant Diseases of viral, viroid, Mycoplasma and uncertain etiology- Karl.Maramarosch. Oxford & IBH Publishing Co. Pvt. Ltd. New Delhi, Bombay, Calcutta.

5.     An Introduction to Mycology (III edition) Alexopopulos CJ and Mims CW, Wiley Eastern Ltd., New Delhi, 1979

Academic Year: 
2015-2016
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