Laboratory V

Paper Code: 
BTE 503
Credits: 
02
Contact Hours: 
60
Objective: 

This course will enable the students to -

  1.  To get acquainted to the cell culture laboratories
  2. Learn the sterilization techniques and their importance
  3. Learn the cell culture media and tissue culture techniques

Course Outcomes (COs): 

Course

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BTE 503

Laboratory V

CO 56To acquire knowledge of a small and middle size plant tissue culture laboratory.

CO 57To learn various techniques of sterilization of the laboratory, glassware, equipment, media, tissues etc.

CO 58To understand the procedure of making plant tissue culture media and stocks

CO 59To have a practical knowledge of explant sterilization and inoculation.

CO 60To study callus induction, embryo culture, raisin virus free plants through meristem culture.

CO 61To study cell viability and enumeration of cells through hemocytometer.

Approach in teaching:

Interactive Lectures, Discussion, Practical experiments

 

Learning activities for the students:

Self learning assignments, Effective questions, Practical experiments in lab, Giving tasks.

 

C A test, Semester end examinations, Viva Voce, Practical record book, Assignments, Classroom interaction

 

CONTENTS:

  1. Introduction to Plant Tissue Culture and lay out of Plant tissue culture Laboratory
  2. Aseptic manipulation techniques
  3. Sterilization of Seeds
  4. Sterilization of Buds, Leaf, Stem , Roots, Tubers, Scales
  5. Preparation of MS medium
  6. Callus Initiation from Arachis hypogea
  7. Multiple shoot proliferation in Citrus nodal segment
  8. Callus differentiation and organogenesis
  9. Introduction to Animal Cell Culture and lay out of Animal Cell Culture Laboratory
  10. Hemocytometry
  11. Cell viability
  12. Apical meristem culture to raise virus free plants
  13. Production of Rhizobium as Biofertiliser

 

Academic Year: 
2020-2021
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