to enable students to-
Course Outcomes (COs):
Course |
Learning outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
---|---|---|---|---|
Paper Code |
Paper Title |
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MBL 121
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Bacteriology
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Upon completion of the course students will be able to CO 1: Inculcate knowledge about structure of bacterial cell. CO 2: Assess major characteristics of Eubacteria and Archaebacteria. CO 3: Gain insight on microbial growth and preservation. CO 4: Evaluate and interpret different classification systems according to Bergey’s manual. CO 5: Understand chemotaxonomy and genetic methods of taxonomy . |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching
Learning activities for the students: Self-learning assignments, Effective questions, Seminar presentation, Giving tasks
|
Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation |
Cell wall structure and synthesis, cell membrane,. Flagella and motility, chemotaxis Pili, Cell inclusions like Glycogen granules, Volutin granules, Carboxysomes etc, Slime sheet and capsule.
Endospre structure and formation, stages of sporulation, activation germination and outgrowth of bacterial endospres
General characters and structure of Spirochetes, cyanobacteria, purple and green bacteria, rickettsia, Chlamydia and mycoplasma
Gram positive bacteria- endospore forming bacteria, actinomycetes, mycobacteria. Archaebacteria-methanotrophs, halophiles and sulphur bacteria.
The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yields, Synchronous growth, Continuous, Batch and Fed Batch Culture; Growth as affected by environmental factors like temperature, pH , activity of water and gaseous environment, maintenance and preservation of microorganisms
Classification of microorganisms-introduction,Haekels three kingdom concept- Whittaker’s five kingdom concept-three domain concept of Carl Woese Characteristics of primary domains
Classification and salient features of bacteria according to Bergey’s manual of systematic bacteriology(a brief outline)
Morphlogical types of bacteria
Nutritional classification of bacteria
Chemotaxonomy (cell wall components, isoprenoid - quinones, amino acid sequence of
proteins, protein profiles, cytochrome composition , ribosomal RNA, etc)
Genetic methods in taxonomy (PCR and DNA Fingerprinting as Identification Tools for
Bacteria, DNA base composition and hybridisation, nucleic acid hybridization, ribotyping; Ribosomal RNA sequencing, molecular chronometers)
Culture collections
Foundation in Microbiology, K P Talaro and A Talaro, (4th edition), Mc Graw Hill, 2002
General Microbiology, R Y Stanier, J L Ingharam, M L Wheelies, P R Painter, Mac Millan Education Ltd, 1999
Laboratory Fundamentals of Microbiology, I E Alcamo, Jones and Barlett publishers, 2001
Microbial Diversity, D Colwd, Academic press, 1999.
Microbiology , M. J Pelczar, E C S Chan, N R Kreig, (5th edition), Tata Mc Graw Publication, 2006
Prokaryotic Development, Y V Burn. & L J Shimkets, ASM Press, 2000