· understand aseptic techniques
· learn classification and structure of microorganisms
· have an idea about microbial growth and factors affecting it
· understand fermentation technology and learn it’s industrial applications
Development of Microbiology (contribution of pioneers).
Concept of sterilization, methods of sterilization-dry heat, wet heat, radiation, filtration and chemicals.
Introduction to different types of microbes: bacteria, viruses, algae, fungi and protozoa structure of bacterial cell- cell membrane, cell wall, flagella and motility, cell inclusion bodies, Sporulation in bacteria.
Nutritional classification of Microorganisms.
Microbial growth, growth curve, factors affecting microbial growth (pH, temperature, oxygen and water availability).
General concepts of industrial microbiology-raw materials used in media production, basic design of a bioreactor; CSTR and Fluidized bed fermentor
Modes of fermentation- batch, continuous and fed batch.
Industrial enzymes-amylases, production of antibiotics-penicillin, amino acid production- glutamic acid, vitamins-riboflavin, industrial alcohol.