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LABORATORY II (MICROBIOLOGY)
The objective of this laboratory course is to provide practical skills on basic microbiological techniques. The students will be able to examine various aseptic methods, design media and formulate experiments for isolation, identification and enumeration of microbes present in a sample. They will be able to analyse the results of antimicrobial sensitivity tests and preserve bacterial cultures.
Sterilization, disinfection and safety in microbiological laboratory.
2. Preparation of media for cultivation of bacteria.
3. Isolation of bacteria in pure culture by streak plate method.
4. Maintenance of stock cultures: slants, stabs and glycerol stock cultures
5. Study of colony and growth characteristics of some common bacteria: Bacillus, E. coli, Staphylococcus, Streptococcus, etc.
6. Preparation of bacterial smear and Staining methods: Negative, Gram’s staining, endospore staining.
7. Enumeration of bacteria: standard plate count.
8. Biochemical characterization of microbes.
9. Isolation and identification of bacteria from soil/water samples.
10. Antimicrobial sensitivity test and demonstration of drug resistance.
11. Determination of phenol co-efficient of antimicrobial agents.
12. Determination of Minimum Inhibitory Concentration (MIC)
13. Any other practical based on theory syllabus.
Cappuccino, J. G., & Welsh, C. (2016). Microbiology: a Laboratory Manual. Benjamin-
Cummings Publishing Company.
2. Collins, C. H., Lyne, P. M., Grange, J. M., & Falkinham III, J. (2004). Collins and Lyne’s
Microbiological Methods (8th ed.). Arnolds.
3. Tille, P. M., & Forbes, B. A.(2015) . Bailey & Scott’s Diagnostic Microbiology(15th edition).
Elsevier Inc
