MOLECULAR BIOLOGY AND MICROBIAL GENETICS

Paper Code: 
MBL 301
Credits: 
03
Contact Hours: 
45
Objective: 

Course Outcomes (COs):

 

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Upon completion of the course learners will be able to:

CO 31: assess the DNA structure and process of replication

CO 32: examine the process of transcription and it’s regulation

CO 33: Interpret the mechanism of translation and regulation of gene expression     

CO 34:  identify and interpret molecular mechanism of mutations and DNA repair

CO 35:evaluate the mechanism of genetic transfers in microbes

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

10.00
Unit I: 
DNA Structure and Replication
  • DNA as a genetic material, structure of DNA and RNA, Types of DNA (A, B, Z), DNA replication in prokaryotes and eukaryotes.

 

8.00
Unit II: 
Transcription
  • Mechanism of Transcription in prokaryotes and eukaryotes, enzymes and transcription factors, Posttranscriptional modifications of eukaryotic mRNA.

 

10.00
Unit III: 
Translation and Regulation of Gene Expression
  • The genetic code, Translational machinery in eukaryotes and prokaryotes, inhibitors of protein synthesis
  • Regulation of Gene expression in prokaryotes (lac and trp operon)

 

9.00
Unit IV: 
Mutations and Mutant Selection
  • Molecular mechanism of mutation, forward and reverse mutation, transition, transversion, Spontaneous and induced mutations
  • Methods for mutant selection- direct selection, antibiotic selection, replica plating
  • Mechanism of DNA repair-photo reactivation, Nucleotide excision repair, mismatch repair

 

8.00
Unit V: 
Recombination in Bacteria
  • Transformation, transduction and conjugation
  • A brief idea of cloning vectors (plasmids, cosmids, bacteriophages).

 

ESSENTIAL READINGS: 
  • Principles of Genetics, 8th edition, Gardener E J, John Wiley and Sons Publications, 2006.
  • Concepts of Genetics, 11th edition, W S Klug, M R Cummings, C A Spencer, M A Palladino, Case Pearson Education, Inc., 2019
  • Lewin’s Genes X, 10th edition, J E Krebs, E Goldstein, S T Kilpatrick, Jones &Barlett Publishers, 2009
  • Cell and Molecular Biology, 8th edition, Gerald Karp, John Wiley and Sons. 2018.
  • Biochemistry and Molecular Biology, 4rd edition, Elliott W H and Elliot D C, Oxford University Press, 2009.
  • Microbial Genetics, 2nd edition, Stanley R Maloy, John E Cronnan, David Freifelder. Jones and Barlett Publishers, 2008.

 

REFERENCES: 

SUGGESTED READINGS:

  • Brock Biology of Microorganisms, 14th edition M T Madigan, J M Martinko, K S Bender, D H Buckley, D A Stahl, Pearson Education, Inc., 2017
  • Essentials of Molecular Biology, 4rd edition, Malacinski G M and Freifelder D Jones and Bartlett Publishers, 2015.
  • Instant Notes, Molecular Biology, 4rd edition, P G Turner, Mc Lennan, A D  Bates, and M R H White, Bios Scientific Publishers. 2016.
  • Cell and Molecular Biology, 3rd edition, Philip Scheeler and Donald E Bianchi, John Wiley and Sons Publications, 2009.

 

e RESOURCES:

JOURNALS:

  • Nature Reviews Molecular Cell Biology
  • Nature Reviews Genetics
  • Molecular Cell
  • Cell Host and Microbe
  • EMBO Journal
  • Molecular Biology and Evolution
  • Microbiology and Molecular Biology Review

 

Academic Year: 
2022-2023
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